| AMGEN SAFETY NET FOUNDATION | Thousand Oaks, CA | $2,729,907,609 | 2024 | G51 | published | |
| TEMPUS UNLIMITED INC | Stoughton, MA | $2,236,284,366 | 2024 | G23 | published | |
| WORLD FEDERATION OF HEMOPHILIA USA | Albany, NY | $1,095,526,264 | 2024 | G20 | published | science: research education |
| AMERICAN HEART ASSOCIATION INC | Dallas, TX | $1,070,192,712 | 2024 | G43 | published | survivor: cancer |
| UNIVERSITY MEDICAL CENTER MANAGEMENT CORPORATON | New Orleans, LA | $792,867,500 | 2024 | G12 | published | abuse: substance |
| FRED HUTCHINSON CANCER RESEARCH CENTER | Seattle, WA | $696,845,474 | 2021 | G30 | inferred 0.85 | survivor: cancer |
| CENTRAL ARKANSAS RADIATION THERAPY INSTITUTE INC | Little Rock, AR | $620,523,795 | 2024 | G30 | published | survivor: cancer |
| BARBARA ANN KARMANOS CANCER HOSPITAL | Detroit, MI | $606,826,459 | 2024 | G30 | published | science: research education |
| DEVEREUX FOUNDATION | Villanova, PA | $507,919,260 | 2024 | G05 | published | abuse: substance |
| CAN COMMUNITY HEALTH INC | Tampa, FL | $506,024,240 | 2024 | G81 | published | |
| ALZHEIMERS DISEASE AND RELATED DISORDERS ASSOCIATION INC | Chicago, IL | $457,244,898 | 2024 | G83 | published | launch: launch |
| LIFESPAN PHYSICIAN GROUP INC | Providence, RI | $366,760,724 | 2024 | G43 | published | abuse: substance |
| BLOOD CANCER UNITED INC | Washington, DC | $358,698,504 | 2024 | G30 | published | survivor: cancer |
| CYSTIC FIBROSIS FOUNDATION | Bethesda, MD | $355,798,320 | 2024 | G20 | published | science: research education |
| AMERICAN KIDNEY FUND INC | Rockville, MD | $324,644,050 | 2025 | G99 | inferred 0.70 | science: research education |
| H LEE MOFFITT CANCER CENTER AND RESEARCH INSTITUTE INC | Tampa, FL | $322,301,135 | 2024 | G05 | published | science: research education |
| MARY BIRD PERKINS CANCER CENTER | Baton Rouge, LA | $318,045,658 | 2024 | G30 | published | survivor: cancer |
| MAXAID | Seattle, WA | $309,119,754 | 2024 | G19 | published | |
| BREAKTHROUGH T1D | New York, NY | $301,723,973 | 2024 | G80 | published | launch: religious |
| DAP HEALTH INC | Palm Springs, CA | $280,264,706 | 2024 | G81 | published | survivor: cancer |
| UNITED CEREBRAL PALSY ASSOCIATIONS OF NEW YORK STATE INC | New York, NY | $274,118,557 | 2024 | G20 | published | men: issues |
| UNITED CEREBRAL PALSY OF NEW YORK CITY INC | New York, NY | $242,730,314 | 2024 | G20 | published | |
| SIGHT SAVERS INTERNATIONAL INC | Boston, MA | $216,230,800 | 2025 | G41 | published | |
| VALLEY CHILDRENS MEDICAL GROUP | Madera, CA | $211,413,193 | 2024 | G98 | published | |
| STOWERS RESOURCE MANAGEMENT INC | Kansas City, MO | $198,300,289 | 2024 | G11 | published | science: research education |
| C H M C ANESTHESIA FOUNDATION INC | Boston, MA | $190,841,798 | 2024 | G90 | published | science: research education |
| H LEE MOFFITT CANCER CTR & RES INST LIFE TIME CANCER SCRN CTR INC | Tampa, FL | $173,244,422 | 2024 | G30 | published | science: research education |
| CHASE BREXTON HEALTH SERVICES INC | Baltimore, MD | $172,818,534 | 2024 | G81 | published | |
| THE CENTER FOR DISCOVERY INC | Harris, NY | $171,818,211 | 2025 | G20 | published | |
| THE RARE DISEASE CHARITABLE FOUNDATION | Lexington, MA | $162,843,174 | 2022 | G80 | inferred 0.60 | |
| NATIONAL MULTIPLE SCLEROSIS SOCIETY | New York, NY | $161,966,823 | 2024 | G50 | published | men: issues |
| AMERICAN SOCIETY OF CLINICAL ONCOLOGY | Alexandria, VA | $159,100,585 | 2024 | G9X | published | survivor: cancer |
| AMERICAN DIABETES ASSOCIATION INC | Arlington, VA | $152,241,578 | 2024 | G80 | published | shelter: disaster |
| AMERICAN COLLEGE OF RADIOLOGY | Reston, VA | $141,352,754 | 2024 | G03 | published | science: research education |
| CARE RESOURCE COMMUNITY HEALTH CENTERS INC | Miami, FL | $138,789,620 | 2024 | G81 | published | |
| AMERICAN ACADEMY OF PEDIATRICS INC | Itasca, IL | $138,498,070 | 2024 | G98 | published | violence: prevention |
| AMERICAN CANCER SOCIETY INC | Atlanta, GA | $136,826,348 | 2024 | G30 | published | survivor: cancer |
| MAKE- A- WISH FOUNDATION OF AMERICA | Phoenix, AZ | $136,767,956 | 2024 | G80 | inferred 0.60 | |
| CANADIAN CANCER SOCIETY | Canada | $134,749,308 | 2024 | G30 | published | survivor: cancer |
| MIAMI CANCER INSTITUTE AT BAPTIST HEALTH INC | Coral Gables, FL | $132,288,902 | 2024 | G30 | published | survivor: cancer |
| AMEGO INC | Attleboro, MA | $125,913,827 | 2024 | G84 | published | |
| AMERICAN COLLEGE OF CARDIOLOGY FOUNDATION | Washington, DC | $125,831,256 | 2024 | G43 | published | science: research education |
| CENTER FOR DISABILITY SERVICES INC | Albany, NY | $125,771,990 | 2024 | G20 | published | women: issues |
| AIDS ARMS INC | Dallas, TX | $124,939,996 | 2024 | G81 | published | women: issues |
| UPSTATE CEREBRAL PALSY INC | Utica, NY | $124,592,778 | 2024 | G20 | published | |
| QSAC INC | Nyc, NY | $120,852,163 | 2024 | G84 | published | |
| EHS INC | Buffalo, NY | $119,895,161 | 2024 | G81 | published | |
| THE AGAINST MALARIA FOUNDATION | Kansas City, MO | $119,395,632 | 2024 | G12 | published | |
| AMERICAN LUNG ASSOCIATION | Chicago, IL | $118,682,229 | 2024 | G45 | published | launch: launch |
| MICHIGAN HEALTH ENDOWMENT FUND | Brighton, MI | $116,075,631 | 2024 | G12 | published | abuse: elder |
| CHILDRENS ANESTHESIOLOGY ASSOCIATES LTD | Philadelphia, PA | $115,647,675 | 2024 | G91 | published | science: research education |
| CYSTIC FIBROSIS FOUNDATION - GROUP RETURN FOR | Bethesda, MD | $113,971,674 | 2015 | G12 | inferred 0.90 | science: research education |
| WASHINGTON INSTITUTE FOR COAGULATION | Seattle, WA | $112,675,357 | 2024 | G99 | published | |
| AMERICAN SOCIETY OF HEMATOLOGY INC | Washington, DC | $112,402,449 | 2024 | G43 | published | science: research education |
| VIVENT HEALTH INC | Milwaukee, WI | $109,460,230 | 2024 | G81 | published | men: issues |
| AMERICAN BOARD OF INTERNAL MEDICINE | Philadelphia, PA | $108,351,701 | 2024 | G95 | published | science: research education |
| CARES COMMUNITY HEALTH | Sacramento, CA | $105,005,933 | 2024 | G81 | published | abuse: substance |
| EYE CARE FOR KIDS | Midvale, UT | $104,247,404 | 2025 | G41 | published | |
| SUSAN G KOMEN BREAST CANCER FOUNDATION | Dallas, TX | $103,154,379 | 2024 | G32 | published | survivor: cancer |
| FUND FOR PUBLIC HEALTH IN NEW YORK INC | New York, NY | $98,316,171 | 2024 | G80 | published | women: issues |
| COMMUNITY SERVICES FOR AUTISTIC ADULTS AND CHILDREN | Montgomry Vlg, MD | $95,701,175 | 2024 | G84 | published | |
| BENEVOLENT HEALTHCARE FOUNDATION | Centennial, CO | $95,701,149 | 2024 | G19 | published | |
| American Cancer Society Inc Eastern | White Plains, NY | $93,589,588 | 2010 | G30 | inferred 0.85 | survivor: cancer |
| AMERICAN CANCER SOCIETY INC HIGH PLAINS | Austin, TX | $92,872,717 | 2011 | G30 | inferred 0.90 | survivor: cancer |
| EQUITAS HEALTH INC | Columbus, OH | $86,300,739 | 2024 | G81 | published | |
| MARCH OF DIMES INC | Arlington, VA | $85,191,655 | 2025 | G20 | published | abuse: substance |
| CROHNS & COLITIS FOUNDATION INC | New York, NY | $85,182,348 | 2024 | G70 | published | science: research education |
| MINNESOTA MEDICAL FOUNDATION | Minneapolis, MN | $79,237,432 | 2011 | G99 | inferred 0.50 | science: research education |
| SIOUXLAND REGIONAL CANCER CENTER | Sioux City, IA | $78,004,916 | 2024 | G30 | published | |
| AIDS CARE GROUP | Chester, PA | $77,454,293 | 2024 | G81 | published | |
| TRENTON MEDICAL CENTER INC | High Springs, FL | $77,329,879 | 2024 | G30 | published | |
| JOSLIN DIABETES CENTER INC | Charlestown, MA | $77,259,482 | 2024 | G80 | published | girls boys: boys |
| NASHVILLE C A R E S INC | Nashville, TN | $75,232,922 | 2024 | G81 | published | science: research education |
| American Cancer Society Inc Great West | Seattle, WA | $74,151,554 | 2011 | G30 | inferred 0.80 | survivor: cancer |
| HIV-AIDS ALLIANCE FOR REGION TWO | Baton Rouge, LA | $73,988,017 | 2024 | G81 | published | abuse: substance |
| ARTHRITIS FOUNDATION INC | Atlanta, GA | $70,127,435 | 2025 | G51 | published | science: research education |
| RADIOLOGICAL SOCIETY OF NORTH AMERICA INC | Oak Brook, IL | $68,473,609 | 2024 | G03 | published | science: research education |
| FEDERATION OF STATE MEDICAL BOARDS OF THE UNITED STATES INC | Euless, TX | $67,031,105 | 2024 | G05 | published | science: research education |
| HONORBRIDGE | Greenville, NC | $66,151,836 | 2025 | G44 | published | |
| UNITED CEREBRAL PALSY ASSOCIATION OF GREATER SUFFOLK INC | Hauppauge, NY | $64,778,495 | 2025 | G20 | published | |
| MUSCULAR DYSTROPHY ASSOCIATION | Chicago, IL | $64,160,032 | 2025 | G50 | published | science: research education |
| AMERICAN CANCER SOCIETY INC MID-SOUTH | Birmingham, AL | $64,151,507 | 2011 | G30 | inferred 0.90 | survivor: cancer |
| BRIGHTFOCUS FOUNDATION | Clarksburg, MD | $63,423,924 | 2024 | G00 | published | launch: launch |
| WHITMAN-WALKER CLINIC INC | Washington, DC | $62,406,560 | 2024 | G81 | published | violence: community |
| THE OAK CLINIC | Green, OH | $62,213,011 | 2024 | G50 | published | |
| OPTICAL SOCIETY OF AMERICA INC | Washington, DC | $61,827,324 | 2024 | G03 | published | science: research education |
| LOUISIANA ORGAN PROCUREMENT AGENCY | Covington, LA | $60,534,270 | 2024 | G44 | published | |
| AMERICAN CANCER SOCIETY INC NEW ENGLAND | Framingham, MA | $58,725,035 | 2011 | G30 | inferred 0.80 | survivor: cancer |
| MAB COMMUNITY SERVICES INC | Brookline, MA | $57,261,156 | 2024 | G41 | published | |
| ACCESSCNY INC | Syracuse, NY | $57,160,218 | 2024 | G23 | published | |
| FOUNDCARE INC | West Palm Bch, FL | $56,778,252 | 2024 | G02 | published | abuse: substance |
| American Cancer Society Inc Midwest Division | Waukesha, WI | $54,430,964 | 2011 | G30 | inferred 0.75 | survivor: cancer |
| HEMOPHILIA OF GEORGIA INC | Sandy Springs, GA | $53,788,955 | 2024 | G20 | published | |
| AURORA INTEGRATED ONCOLOGY FOUNDATION | Joelton, TN | $53,740,844 | 2024 | G30 | published | |
| AMERICAN ACADEMY OF DERMATOLOGY INC | Rosemont, IL | $53,579,298 | 2024 | G90 | published | science: research education |
| AMERICAN ACADEMY OF ORTHOPEDIC SURGEONS | Rosemont, IL | $53,567,386 | 2024 | G03 | published | science: research education |
| ECOG-ACRIN MEDICAL RESEARCH FOUNDATION INC | Philadelphia, PA | $53,346,165 | 2024 | G05 | published | science: research education |
| PILLAR CARE CONTINUUM INC | East Hanover, NJ | $52,908,973 | 2024 | G20 | published | |
| NEW JERSEY INSTITUTE FOR DISABILITIES INC | Edison, NJ | $51,334,047 | 2024 | G20 | published | |
| UNITED CEREBRAL PALSY OF CENTRAL FLORIDA INC | Orlando, FL | $50,954,410 | 2024 | G23 | published | |