| NATIONAL FISH AND WILDLIFE FOUNDATION | $584,225,222 | D30 | published | launch: launch | Washington, DC | 2024 |
| AMERICAN SOCIETY FOR THE PREVENTION OF CRUELTY TO ANIMALS | $446,134,125 | D20 | published | survivor: disaster | New York, NY | 2024 |
| SAN DIEGO ZOO WILDLIFE ALLIANCE | $437,607,695 | D50 | published | science: research education | San Diego, CA | 2024 |
| WILDLIFE CONSERVATION SOCIETY | $400,861,377 | D50 | published | domestic: animals | Bronx, NY | 2024 |
| HUMANE WORLD FOR ANIMALS INC | $216,548,337 | D20 | published | domestic: animals | Washington, DC | 2025 |
| MONTEREY BAY AQUARIUM FOUNDATION | $208,135,651 | D11 | published | launch: launch | Monterey, CA | 2024 |
| BEST FRIENDS ANIMAL SOCIETY | $173,780,697 | D20 | published | abuse: animal | Kanab, UT | 2024 |
| JOHNNY MORRIS OZARKS HERITAGE PRESERVE | $167,956,538 | D30 | published | | Springfield, MO | 2024 |
| GEORGIA AQUARIUM INC | $165,667,302 | D50 | published | science: research education | Atlanta, GA | 2024 |
| AHIMSA FOUNDATION | $160,180,393 | D20 | published | | Niwot, CO | 2024 |
| MASSACHUSETTS SOCIETY FOR THE PREVENTION OF CRUELTY TO ANIMALS | $152,387,415 | D20 | published | abuse: animal | Boston, MA | 2025 |
| NATIONAL WILDLIFE FEDERATION | $124,061,599 | D30 | published | domestic: animals | Reston, VA | 2024 |
| PEOPLE FOR THE ETHICAL TREATMENT OF ANIMALS INC | $110,179,122 | D01 | published | domestic: animals | Norfolk, VA | 2024 |
| TROUT UNLIMITED INC | $109,528,553 | D33 | published | launch: launch | Arlington, VA | 2024 |
| SHEDD AQUARIUM SOCIETY | $101,974,802 | D50 | published | science: research education | Chicago, IL | 2024 |
| ROCKY MOUNTAIN ELK FOUNDATION INC | $100,089,143 | D30 | published | science: research education | Missoula, MT | 2024 |
| AMERICAN KENNEL CLUB INC | $99,646,198 | D60 | published | domestic violence | New York, NY | 2024 |
| THE ANIMAL MEDICAL CENTER | $96,735,746 | D40 | published | domestic: animals | New York, NY | 2024 |
| DENVER DUMB FRIENDS LEAGUE | $92,897,062 | D20 | published | abuse: animal | Denver, CO | 2024 |
| PANTHERA CORPORATION | $91,771,873 | D30 | published | science: research education | New York, NY | 2025 |
| CHICAGO ZOOLOGICAL SOCIETY | $90,541,500 | D50 | published | science: research education | Brookfield, IL | 2025 |
| COLUMBUS ZOOLOGICAL PARK ASSOCIATION | $88,819,571 | D50 | published | | Powell, OH | 2024 |
| WILDLIFE CONSERVATION NETWORK INC | $86,052,292 | D30 | published | domestic: animals | San Francisco, CA | 2025 |
| OCEAN HABITAT AND WILDLIFE RESCUE INC | $85,995,963 | D20 | published | | West Palm Bch, FL | 2025 |
| WOODLAND PARK ZOOLOGICAL SOCIETY | $83,715,805 | D50 | published | science: research education | Seattle, WA | 2024 |
| HOUSTON ZOO INC | $82,689,282 | D11 | published | space: outer | Houston, TX | 2024 |
| OMAHA ZOOLOGICAL SOCIETY INC | $82,044,812 | D50 | published | science: research education | Omaha, NE | 2024 |
| GREAT LAKES FISHERY COMMISSION | $80,320,800 | D33 | published | | Ann Arbor, MI | 2024 |
| REWILD | $75,177,871 | D30 | published | science: research education | Austin, TX | 2024 |
| PETSMART CHARITIES INC | $71,189,403 | D12 | published | shelter: animal | Phoenix, AZ | 2025 |
| NASHVILLE ZOO INC | $69,968,685 | D50 | published | science: research education | Nashville, TN | 2025 |
| ZOOLOGICAL SOCIETY OF CINCINNATI | $69,378,528 | D50 | published | | Cincinnati, OH | 2024 |
| SAN DIEGO HUMANE SOCIETY AND SPCA | $67,557,221 | D20 | published | domestic: animals | San Diego, CA | 2024 |
| DENVER ZOOLOGICAL FOUNDATION INC | $66,039,623 | D11 | published | domestic: animals | Denver, CO | 2025 |
| GOVERNORS ISLAND CORPORATION | $65,325,995 | D30 | published | | New York, NY | 2024 |
| DETROIT ZOOLOGICAL SOCIETY | $64,149,877 | D50 | published | abuse: substance | Royal Oak, MI | 2024 |
| ARIZONA CENTER FOR NATURE CONSERVATION | $63,967,380 | D50 | published | | Phoenix, AZ | 2024 |
| FORT WORTH ZOOLOGICAL ASSOCIATION INC | $62,181,894 | D50 | published | | Fort Worth, TX | 2024 |
| AUDUBON NATURE INSTITUTE INC | $61,293,521 | D12 | published | domestic: animals | New Orleans, LA | 2024 |
| SAN ANTONIO ZOOLOGICAL SOCIETY INC | $61,090,159 | D50 | published | | San Antonio, TX | 2024 |
| AQUARIUM OF THE PACIFIC | $59,309,760 | D50 | published | science: research education | Long Beach, CA | 2024 |
| SAN FRANCISCO SOCIETY FOR THE PREVENTION OF CRUELTY TO ANIMALS | $58,331,987 | D20 | published | shelter: animal | San Francisco, CA | 2024 |
| INDIANAPOLIS ZOOLOGICAL SOCIETY INC | $57,679,714 | D50 | published | | Indianapolis, IN | 2024 |
| NEW ENGLAND AQUARIUM CORPORATION | $54,645,914 | D50 | published | space: outer | Boston, MA | 2024 |
| NATIONAL AQUARIUM INC | $54,351,794 | D50 | published | science: research education | Baltimore, MD | 2025 |
| SEATTLE AQUARIUM SOCIETY-SEAS | $54,098,416 | D11 | published | science: research education | Seattle, WA | 2024 |
| THE SAINT LOUIS ZOO FOUNDATION | $53,824,998 | D11 | published | | Saint Louis, MO | 2025 |
| FRIENDS OF THE ZOO INC OF KANSAS CITY MO | $53,626,478 | D11 | published | science: research education | Kansas City, MO | 2024 |
| JACKSONVILLE ZOOLOGICAL SOCIETY INC | $50,606,141 | D50 | published | | Jacksonville, FL | 2024 |
| LINCOLN PARK ZOOLOGICAL SOCIETY | $50,519,973 | D50 | published | science: research education | Chicago, IL | 2024 |
| TOLEDO ZOOLOGICAL SOCIETY | $50,015,283 | D50 | published | | Toledo, OH | 2024 |
| LOWRY PARK ZOOLOGICAL SOCIETY OF TAMPA INC | $48,990,299 | D50 | published | survivor: cancer | Tampa, FL | 2024 |
| DEFENDERS OF WILDLIFE | $48,550,195 | D30 | published | science: research education | Washington, DC | 2024 |
| PAWS CHICAGO | $46,965,874 | D20 | published | abuse: animal | Chicago, IL | 2024 |
| ARIZONA HUMANE SOCIETY | $46,689,046 | D20 | published | abuse: animal | Phoenix, AZ | 2024 |
| NORTH SHORE ANIMAL LEAGUE AMERICA INC | $46,442,949 | D20 | published | shelter: animal | Prt Washingtn, NY | 2024 |
| ANIMAL RELIEF NETWORK | $45,837,086 | D20 | published | | Austin, TX | 2025 |
| INTERNATIONAL FUND FOR ANIMAL WELFARE INC | $44,498,195 | D20 | published | domestic: animals | Yarmouth Port, MA | 2024 |
| ZOOLOGICAL SOCIETY OF PHILADELPHIA | $43,711,192 | D50 | published | domestic: animals | Philadelphia, PA | 2024 |
| CENTER FOR BIOLOGICAL DIVERSITY INC | $42,909,696 | D20 | published | science: research education | Tucson, AZ | 2025 |
| ATLANTA-FULTON COUNTY ZOO INC | $42,311,626 | D50 | published | domestic: animals | Atlanta, GA | 2025 |
| HUMANE SOCIETY OF TAMPA BAY INC | $42,054,267 | D20 | published | abuse: animal | Tampa, FL | 2025 |
| ANIMAL CARE AND CONTROL OF NEW YORK CITY INC | $41,655,330 | D20 | published | abuse: animal | New York, NY | 2024 |
| BIRDLIFE INTERNATIONAL | $41,292,150 | D32 | published | | United Kingdom | 2024 |
| BRANDYWINE VALLEY SOCIETY FOR THE PREVENTION OF CRUELTY TO ANIMALS | $39,677,691 | D20 | published | abuse: animal | West Chester, PA | 2025 |
| THE WILD ANIMAL SANCTUARY A COLORADO NON-PROFIT CORPORATION | $38,304,328 | D34 | published | abuse: animal | Keenesburg, CO | 2025 |
| OREGON HUMANE SOCIETY | $37,463,888 | D20 | published | abuse: animal | Portland, OR | 2025 |
| JOHN BALL ZOO | $37,349,675 | D11 | published | science: research education | Grand Rapids, MI | 2024 |
| AFRICAN WILDLIFE FOUNDATION | $37,344,195 | D30 | published | domestic: animals | Washington, DC | 2024 |
| FLORIDA AQUARIUM INC | $37,200,637 | D50 | published | girls boys: girls | Tampa, FL | 2024 |
| DALLAS ZOO MANAGEMENT INC | $36,852,417 | D11 | published | science: research education | Dallas, TX | 2024 |
| AMERICAN HUMANE ASSOCIATION | $36,567,566 | D20 | published | shelter: disaster | Washington, DC | 2024 |
| DOVE LEWIS EMERGENCY ANIMAL HOSPITAL INC | $35,964,307 | D40 | published | domestic: animals | Portland, OR | 2024 |
| HUMANE WORLD FOR ANIMALS INTERNATIONAL | $35,136,528 | D20 | published | domestic: animals | Washington, DC | 2024 |
| CONSERVATION SOCIETY OF CALIFORNIA | $35,008,424 | D50 | published | domestic: animals | Oakland, CA | 2024 |
| CHEYENNE MOUNTAIN ZOOLOGICAL SOCIETY | $34,386,132 | D50 | published | | Colorado Spgs, CO | 2025 |
| COMMONWEALTH ZOOLOGICAL CORPORATION | $34,370,304 | D50 | published | science: research education | Boston, MA | 2024 |
| KARUNA FOUNDATION | $34,225,206 | D01 | published | | Cheyenne, WY | 2025 |
| TENNESSEE AQUARIUM | $34,216,548 | D50 | published | science: research education | Chattanooga, TN | 2024 |
| SEA RESEARCH FOUNDATION INC | $34,034,517 | D50 | published | space: outer | Mystic, CT | 2025 |
| MULE DEER FOUNDATION | $33,166,362 | D30 | published | | Clearfield, UT | 2024 |
| DANE COUNTY HUMANE SOCIETY | $31,846,259 | D20 | published | girls boys: girl scouts | Madison, WI | 2025 |
| MICHIGAN HUMANE SOCIETY | $31,829,647 | D20 | published | domestic: animals | Detroit, MI | 2024 |
| POINT REYES BIRD OBSERVATORY | $31,721,037 | D32 | published | science: research education | Petaluma, CA | 2024 |
| HUMANE SOCIETY OF MISSOURI | $31,581,705 | D20 | published | shelter: disaster | Saint Louis, MO | 2024 |
| FOUNDATION TO SUPPORT ANIMAL PROTECTION | $30,958,377 | D12 | published | science: research education | Norfolk, VA | 2024 |
| EAST COAST ZOOLOGICAL SOCIETY OF FLORIDA INC | $30,844,454 | D50 | published | | Melbourne, FL | 2024 |
| NEW ENGLAND WILDLIFE CENTER | $30,843,812 | D34 | published | | S Weymouth, MA | 2024 |
| MEMPHIS ZOO INC | $30,584,120 | D50 | published | | Memphis, TN | 2024 |
| JANE GOODALL INSTITUTE FOR WILDLIFE RESEARCH EDUCATION & CONSERVATION | $30,550,365 | D05 | published | violence: sexual | Washington, DC | 2024 |
| ZOOLOGICAL SOCIETY OF PITTSBURGH | $30,459,605 | D50 | published | science: research education | Pittsburgh, PA | 2024 |
| WVC | $30,179,624 | D02 | published | | Las Vegas, NV | 2025 |
| FLORIDA VETERINARY MEDICINE FACULTY ASSOCIATION INC | $29,915,803 | D03 | published | science: research education | Gainesville, FL | 2024 |
| FRESNOS CHAFFEE ZOO CORPORATION | $29,607,528 | D50 | published | | Fresno, CA | 2024 |
| PENINSULA HUMANE SOCIETY & SPCA | $28,134,220 | D20 | published | abuse: animal | Burlingame, CA | 2024 |
| AMERICAN BIRD CONSERVANCY | $26,955,379 | D01 | published | science: research education | Marshall, VA | 2024 |
| ZOOLOGICAL SOCIETY OF THE PALM BEACHES INC | $26,669,019 | D50 | published | science: research education | West Palm Bch, FL | 2024 |
| OMAHA ZOO FOUNDATION | $26,326,840 | D30 | published | | Omaha, NE | 2024 |
| EMANCIPET | $26,160,953 | D20 | published | | Austin, TX | 2024 |
| FISH & WILDLIFE FOUNDATION OF FLORIDA INC | $25,766,143 | D12 | published | girls boys: girl scouts | Tallahassee, FL | 2024 |