| NATIONAL FISH AND WILDLIFE FOUNDATION | $584,225,222 | D30 | published | launch: launch | Washington, DC | 2024 |
| JOHNNY MORRIS OZARKS HERITAGE PRESERVE | $167,956,538 | D30 | published | | Springfield, MO | 2024 |
| NATIONAL WILDLIFE FEDERATION | $124,061,599 | D30 | published | domestic: animals | Reston, VA | 2024 |
| ROCKY MOUNTAIN ELK FOUNDATION INC | $100,089,143 | D30 | published | science: research education | Missoula, MT | 2024 |
| PANTHERA CORPORATION | $91,771,873 | D30 | published | science: research education | New York, NY | 2025 |
| WILDLIFE CONSERVATION NETWORK INC | $86,052,292 | D30 | published | domestic: animals | San Francisco, CA | 2025 |
| REWILD | $75,177,871 | D30 | published | science: research education | Austin, TX | 2024 |
| GOVERNORS ISLAND CORPORATION | $65,325,995 | D30 | published | | New York, NY | 2024 |
| DEFENDERS OF WILDLIFE | $48,550,195 | D30 | published | science: research education | Washington, DC | 2024 |
| AFRICAN WILDLIFE FOUNDATION | $37,344,195 | D30 | published | domestic: animals | Washington, DC | 2024 |
| MULE DEER FOUNDATION | $33,166,362 | D30 | published | | Clearfield, UT | 2024 |
| OMAHA ZOO FOUNDATION | $26,326,840 | D30 | published | | Omaha, NE | 2024 |
| ROCKY MOUNTAIN ELK FOUNDATION INC GROUP RETURN | $25,237,191 | D30 | inferred 0.60 | | Missoula, MT | 2024 |
| DELTA WATERFOWL FOUNDATION | $23,616,795 | D30 | published | science: research education | Bismarck, ND | 2024 |
| MARINE MAMMAL CENTER | $18,607,476 | D30 | published | science: research education | Sausalito, CA | 2024 |
| SAFARI CLUB INTERNATIONAL FOUNDATION | $18,605,009 | D30 | published | girls boys: boy scouts | San Antonio, TX | 2024 |
| ODC NETWORK | $18,158,066 | D30 | published | girls boys: boys girls clubs | Holland, MI | 2025 |
| THE FOUNDATION FOR NORTH AMERICA WILD SHEEP | $14,811,770 | D30 | published | science: research education | Cody, WY | 2024 |
| WILDCARE | $13,574,877 | D30 | inferred 0.70 | science: research education | San Rafael, CA | 2024 |
| FRANKFURT ZOOLOGICAL SOCIETY US INC | $13,478,170 | D30 | published | | Washington, DC | 2025 |
| WILDLIFE MISSISSIPPI | $13,244,167 | D30 | published | | Stoneville, MS | 2024 |
| WAGS PET CLINIC INC | $11,794,058 | D30 | published | | Tampa, FL | 2025 |
| THEODORE ROOSEVELT CONSERVATION PARTNERSHIP INC | $11,546,406 | D30 | published | launch: launch | Washington, DC | 2025 |
| PEREGRINE FUND INC | $11,365,825 | D30 | inferred 0.80 | science: research education | Boise, ID | 2024 |
| CALIFORNIA DEER ASSOCIATION | $11,322,913 | D30 | published | | Redding, CA | 2023 |
| TURTLE SURVIVAL ALLIANCE | $11,011,840 | D30 | published | science: research education | N Charleston, SC | 2025 |
| CAESAR KLEBERG FOUNDATION FOR WILDLIFE CONSERVATION | $9,376,415 | D30 | inferred 0.60 | | Kingsville, TX | 2024 |
| ALASKA WILDLIFE CONSERVATION CENTER INC | $8,678,327 | D30 | published | shelter: animal | Girdwood, AK | 2025 |
| ROCKY MOUNTAIN BUTTERFLY CONSORTIUM | $8,279,543 | D30 | published | | Westminster, CO | 2025 |
| PINOLA CONSERVANCY | $7,978,192 | D30 | published | | Shreveport, LA | 2024 |
| HAWK MOUNTAIN SANCTUARY ASSOCIATION | $7,604,944 | D30 | published | science: research education | Kempton, PA | 2024 |
| GRAND SLAM CLUB NORTH AMERICAN SHEEP HUNTERS ASSOCIATION | $7,269,059 | D30 | published | | Birmingham, AL | 2024 |
| BUBYE VALLEY CONSERVATION RESEARCH | $7,260,177 | D30 | published | | Pleasanton, CA | 2024 |
| FRIENDS OF WILDCARE | $6,149,980 | D30 | inferred 0.60 | | San Rafael, CA | 2023 |
| ALABAMA WILDLIFE FEDERATION INC | $5,467,729 | D30 | published | | Millbrook, AL | 2024 |
| FRIENDS OF BIRDLIFE INTERNATIONAL INC | $5,375,929 | D30 | published | | New York, NY | 2024 |
| THE WYLDLIFE FUND | $5,351,996 | D30 | published | women: issues | Buffalo, WY | 2025 |
| GREEN AMERICA | $5,230,441 | D30 | published | | Washington, DC | 2024 |
| WHITE BUFFALO INC | $5,212,286 | D30 | published | | Chester, CT | 2024 |
| WYSOCKING WILDLIFE SANCTUARY INC | $5,160,388 | D30 | published | | Engelhard, NC | 2024 |
| INTERNATIONAL ORDER OF T ROOSEVELT | $5,103,822 | D30 | published | | Marshfield, WI | 2023 |
| BOONE & CROCKETT CLUB | $5,053,864 | D30 | published | girls boys: boy scouts | Missoula, MT | 2024 |
| GREAT PLAINS CONSERVATION FOUNDATION | $4,996,049 | D30 | published | launch: launch | Jupiter, FL | 2024 |
| SMALL WILD CAT CONSERVATION FOUNDATION | $4,915,000 | D30 | published | | Monte Sereno, CA | 2025 |
| INTERNATIONAL SEAFOOD SUSTAINABILITY FOUNDATION | $4,798,588 | D30 | published | science: research education | Pittsburgh, PA | 2025 |
| LINDA RAMBLER CONSERVANCY TRUST | $4,760,758 | D30 | published | | Hershey, PA | 2025 |
| CALIFORNIA STATE WILDLIFE FOUNDATION | $4,707,220 | D30 | published | space: outer | Berkeley, CA | 2024 |
| OREGON WILDLIFE HERITAGE FOUNDATION | $4,705,963 | D30 | published | | Portland, OR | 2024 |
| GREATER FARALLONES ASSOCIATION | $4,566,548 | D30 | published | science: research education | San Francisco, CA | 2024 |
| PRESERVATION OF AT-RISK WILDLIFE PAW FOUNDATION | $4,526,114 | D30 | published | | Boston, MA | 2024 |
| CALIFORNIA MARINE SANCTUARY FOUNDATION | $4,452,900 | D30 | published | science: research education | Monterey, CA | 2024 |
| LEWA WILDLIFE CONSERVANCY USA | $4,393,471 | D30 | published | | New York, NY | 2025 |
| CALIFORNIA LAND STEWARDSHIP INSTITUTE | $4,160,837 | D30 | published | | Napa, CA | 2025 |
| INTERNATIONAL BIRD RESCUE | $4,063,693 | D30 | published | science: research education | Fairfield, CA | 2024 |
| CLINIC FOR THE REHABILITATION OF WILDLIFE INC | $3,940,193 | D30 | published | science: research education | Sanibel, FL | 2024 |
| AMPHIBIAN AND REPTILE CONSERVANCY INC | $3,569,313 | D30 | published | science: research education | Louisville, KY | 2024 |
| TUCSON AUDUBON SOCIETY INC | $3,553,748 | D30 | published | science: research education | Tucson, AZ | 2024 |
| MARINE MAMMAL CARE CENTER LOS ANGELES | $3,517,693 | D30 | published | science: research education | San Pedro, CA | 2025 |
| WILDLIFE HABITAT COUNCIL INC | $3,516,103 | D30 | published | science: research education | Bethesda, MD | 2024 |
| ONCAFARI INTERNATIONAL INC | $3,431,609 | D30 | published | science: research education | Miami, FL | 2025 |
| ELEPHANT HAVENS WILDLIFE FOUNDATION INC | $3,402,691 | D30 | published | | Dallas, TX | 2025 |
| TURTLE CONSERVANCY | $3,309,320 | D30 | published | science: research education | Ojai, CA | 2024 |
| PAPAHANAUMOKUAKEA MARINE DEBRIS PROJECT INC | $3,307,952 | D30 | published | | Kailua, HI | 2024 |
| WILDLIFE CONSERVATION FOUNDATION | $3,170,074 | D30 | published | | Roosevelt, UT | 2025 |
| HENRY L HEYMANN WILDLIFE PRESERVATION FOUNDATION | $3,125,703 | D30 | published | | Bronx, NY | 2024 |
| WILD LANDSCAPES INC | $3,078,044 | D30 | published | | Tallahassee, FL | 2024 |
| NORCROSS WILDLIFE FOUNDATION INC | $2,938,077 | D30 | published | science: research education | Wales, MA | 2025 |
| ALASKA ESKIMO WHALING COMMISSION | $2,906,938 | D30 | inferred 0.50 | | Barrow, AK | 2024 |
| CATMOSPHERE FOUNDATION INC | $2,900,000 | D30 | published | women: issues | Sterling, VA | 2025 |
| MOUSEBUTT RESCUE FOUNDATION INC | $2,853,662 | D30 | published | abuse: animal | Los Angeles, CA | 2024 |
| WILDLIFE PRESERVES INCORPORATED | $2,734,895 | D30 | published | girls boys: boy scouts | Parsippany, NJ | 2025 |
| QUAIL FEDERATION INC | $2,637,152 | D30 | published | | Dallas, TX | 2024 |
| WILD AFRICA | $2,485,394 | D30 | published | | Lafayette, CA | 2024 |
| DOLPHIN LIFE INC | $2,483,909 | D30 | published | science: research education | Key Largo, FL | 2024 |
| WILDLIFE GUARDIANS | $2,458,153 | D30 | published | | Washington, DC | 2024 |
| FOCUSED CONSERVATION SOLUTIONS INC | $2,452,187 | D30 | published | | Fleming Island, FL | 2025 |
| PASADENA AUDUBON SOCIETY | $2,420,470 | D30 | published | | Pasadena, CA | 2024 |
| IDAHO FISH AND WILDLIFE FOUNDATION INC | $2,365,935 | D30 | published | | Boise, ID | 2025 |
| SNIP COLLIER INC | $2,097,129 | D30 | published | | Naples, FL | 2025 |
| WING HAVEN FOUNDATION INCORPORATED | $2,053,506 | D30 | published | | Charlotte, NC | 2025 |
| PEACEABLE PRIMATE SANTUARY | $2,046,951 | D30 | published | | Winamac, IN | 2024 |
| UTAH WILD SHEEP FOUNDATION | $2,009,959 | D30 | published | | W Valley City, UT | 2025 |
| UPWELL TURTLES | $1,980,565 | D30 | published | | Monterey, CA | 2025 |
| FOREVER FOUNDATION FOR TEXAS WILDLIFE INC DBA TEXAS WILDLIFE | $1,932,402 | D30 | published | science: research education | New Braunfels, TX | 2024 |
| NEMOURS PLANTATION WILDLIFE FOUNDATION | $1,926,740 | D30 | published | science: research education | Yemassee, SC | 2025 |
| WILDLIFE AND ENVIRONMENTAL CONSERVATION INCORPORATED | $1,916,252 | D30 | published | | Moorpark, CA | 2025 |
| OCEAN CONNECTIONS INC | $1,877,509 | D30 | published | | Nashotah, WI | 2025 |
| WILDLIFE FOREVER | $1,813,535 | D30 | published | science: research education | Saint Paul, MN | 2025 |
| SPACE FOR GIANTS USA INC | $1,789,084 | D30 | published | | New York, NY | 2025 |
| LIBERTY WILDLIFE INC | $1,732,930 | D30 | inferred 0.90 | science: research education | Phoenix, AZ | 2024 |
| OCEAN OUTCOMES | $1,717,307 | D30 | published | science: research education | Portland, OR | 2024 |
| PERMIAN BASIN CONSERVATION FOUNDATION | $1,698,539 | D30 | published | | Austin, TX | 2024 |
| ROLLING PLAINS QUAIL RESEARCHFOUNDATION | $1,686,021 | D30 | published | | Roby, TX | 2024 |
| WILDLIFE HABITAT FEDERATION | $1,678,733 | D30 | published | | Sealy, TX | 2024 |
| CONSERVE WILDLIFE FOUNDATION OF NEW JERSEY INC | $1,599,622 | D30 | published | science: research education | Princeton, NJ | 2024 |
| SYLVAN HEIGHTS WATERFOWL PARK AND ECO-CENTER INC | $1,591,166 | D30 | published | | Scotland Neck, NC | 2024 |
| PAN AFRICAN SANCTUARY ALLIANCE | $1,571,552 | D30 | published | launch: launch | Portland, OR | 2024 |
| FRIENDS OF BONOBOS | $1,549,566 | D30 | published | | Durham, NC | 2024 |
| SMELTS SEA MAMMAL EDUCATION LEARNING TRAINING SOCIETY | $1,542,398 | D30 | published | science: research education | Sedro Woolley, WA | 2024 |
| PRIMARILY PRIMATES INC | $1,533,217 | D30 | published | abuse: animal | San Antonio, TX | 2025 |