| NATURE CONSERVANCY | Arlington, VA | $1,746,229,436 | 2024 | C30 | inferred 0.85 | launch: launch |
| NEW VENTURE FUND | Washington, DC | $662,364,100 | 2024 | C05 | published | violence: gun |
| NATIONAL FISH AND WILDLIFE FOUNDATION | Washington, DC | $584,225,222 | 2024 | D30 | published | launch: launch |
| EAGLE FOUNDATION | Omaha, NE | $447,008,575 | 2024 | C30 | inferred 0.50 | shelter: homeless |
| AMERICAN SOCIETY FOR THE PREVENTION OF CRUELTY TO ANIMALS | New York, NY | $446,134,125 | 2024 | D20 | published | survivor: disaster |
| ENVIRONMENTAL DEFENSE FUND INCORPORATED | New York, NY | $443,267,772 | 2024 | C60 | published | launch: launch |
| SAN DIEGO ZOO WILDLIFE ALLIANCE | San Diego, CA | $437,607,695 | 2024 | D50 | published | science: research education |
| WILDLIFE CONSERVATION SOCIETY | Bronx, NY | $400,861,377 | 2024 | D50 | published | domestic: animals |
| DUCKS UNLIMITED INC | Memphis, TN | $380,340,506 | 2024 | C32 | published | |
| WORLD WILDLIFE FUND INC | Washington, DC | $366,655,209 | 2024 | C30 | published | launch: launch |
| CENTER FOR SUSTAINABLE ENERGY | San Diego, CA | $335,556,775 | 2024 | C35 | published | launch: launch |
| THE CONSERVATION FUND A NONPROFIT CORPORATION | Arlington, VA | $329,373,956 | 2025 | C30 | published | |
| CONSERVATION INTERNATIONAL FOUNDATION | Arlington, VA | $308,673,004 | 2024 | C30 | published | science: research education |
| WINDWARD FUND | Washington, DC | $308,245,754 | 2024 | C30 | published | |
| ISO NEW ENGLAND INC | Holyoke, MA | $303,262,191 | 2025 | C35 | published | science: research education |
| TRUST FOR PUBLIC LAND | San Francisco, CA | $301,245,450 | 2024 | C50 | published | |
| SIXTEEN THIRTY FUND | Washington, DC | $282,241,759 | 2024 | C60 | published | violence: gun |
| CLIMATE IMPERATIVE FOUNDATION | San Francisco, CA | $271,730,022 | 2024 | C12 | published | |
| ENERGY TRUST OF OREGON INC | Portland, OR | $251,243,122 | 2024 | C35 | published | launch: launch |
| NATURAL RESOURCES DEFENSE COUNCIL INC | New York, NY | $248,922,592 | 2024 | C30 | published | launch: launch |
| HUMANE WORLD FOR ANIMALS INC | Washington, DC | $216,548,337 | 2025 | D20 | published | domestic: animals |
| MONTEREY BAY AQUARIUM FOUNDATION | Monterey, CA | $208,135,651 | 2024 | D11 | published | launch: launch |
| NATIONAL AUDUBON SOCIETY INC | New York, NY | $197,300,511 | 2024 | C30 | published | science: research education |
| ROCKY MOUNTAIN INSTITUTE | Boulder, CO | $174,917,694 | 2024 | C34 | inferred 0.60 | |
| BEST FRIENDS ANIMAL SOCIETY | Kanab, UT | $173,780,697 | 2024 | D20 | published | abuse: animal |
| EARTHJUSTICE | San Francisco, CA | $170,827,511 | 2024 | C01 | published | women: issues |
| JOHNNY MORRIS OZARKS HERITAGE PRESERVE | Springfield, MO | $167,956,538 | 2024 | D30 | published | |
| GEORGIA AQUARIUM INC | Atlanta, GA | $165,667,302 | 2024 | D50 | published | science: research education |
| AHIMSA FOUNDATION | Niwot, CO | $160,180,393 | 2024 | D20 | published | |
| MASSACHUSETTS SOCIETY FOR THE PREVENTION OF CRUELTY TO ANIMALS | Boston, MA | $152,387,415 | 2025 | D20 | published | abuse: animal |
| GREAT HEARTS AMERICA-TEXAS | San Antonio, TX | $148,570,155 | 2024 | C27 | published | |
| NATURE CONSERVATION TR | Decatur, IL | $134,751,852 | 2025 | C20 | published | |
| VERMONT ENERGY INVESTMENT CORP | Winooski, VT | $130,939,426 | 2024 | C35 | published | |
| NEW YORK BOTANICAL GARDEN | Bronx, NY | $124,690,916 | 2024 | C41 | published | science: research education |
| NATIONAL WILDLIFE FEDERATION | Reston, VA | $124,061,599 | 2024 | D30 | published | domestic: animals |
| MARSHALL AREA ECONOMIC DEVELOPMENT ALLIANCE | Marshall, MI | $119,254,589 | 2024 | C99 | published | |
| PHEASANTS FOREVER INC | Saint Paul, MN | $118,599,546 | 2024 | C36 | inferred 0.70 | |
| MARINE SPILL RESPONSE CORPORATION | Herndon, VA | $112,384,332 | 2024 | C27 | inferred 0.50 | |
| NATIONAL ARBOR DAY FOUNDATION | Lincoln, NE | $111,866,996 | 2024 | C50 | published | |
| PEOPLE FOR THE ETHICAL TREATMENT OF ANIMALS INC | Norfolk, VA | $110,179,122 | 2024 | D01 | published | domestic: animals |
| TROUT UNLIMITED INC | Arlington, VA | $109,528,553 | 2024 | D33 | published | launch: launch |
| SHEDD AQUARIUM SOCIETY | Chicago, IL | $101,974,802 | 2024 | D50 | published | science: research education |
| RAINFOREST ALLIANCE INC | New York, NY | $100,221,940 | 2024 | C30 | inferred 0.60 | violence: sexual |
| ROCKY MOUNTAIN ELK FOUNDATION INC | Missoula, MT | $100,089,143 | 2024 | D30 | published | science: research education |
| THANKSGIVING POINT INSTITUTE INC | Lehi, UT | $99,872,507 | 2024 | C41 | published | science: research education |
| AMERICAN KENNEL CLUB INC | New York, NY | $99,646,198 | 2024 | D60 | published | domestic violence |
| THE ANIMAL MEDICAL CENTER | New York, NY | $96,735,746 | 2024 | D40 | published | domestic: animals |
| SOCIAL AND ENVIRONMENTAL ENTREPRENEURS INC | Calabasas, CA | $94,599,962 | 2024 | C05 | published | women: issues |
| FOUR LAKES TASK FORCE | Midland, MI | $93,735,697 | 2025 | C32 | published | |
| DENVER DUMB FRIENDS LEAGUE | Denver, CO | $92,897,062 | 2024 | D20 | published | abuse: animal |
| URBAN LAND INSTITUTE | Washington, DC | $92,328,399 | 2024 | C34 | published | women: issues |
| PANTHERA CORPORATION | New York, NY | $91,771,873 | 2025 | D30 | published | science: research education |
| CHICAGO ZOOLOGICAL SOCIETY | Brookfield, IL | $90,541,500 | 2025 | D50 | published | science: research education |
| COLUMBUS ZOOLOGICAL PARK ASSOCIATION | Powell, OH | $88,819,571 | 2024 | D50 | published | |
| WILDLIFE CONSERVATION NETWORK INC | San Francisco, CA | $86,052,292 | 2025 | D30 | published | domestic: animals |
| OCEAN HABITAT AND WILDLIFE RESCUE INC | West Palm Bch, FL | $85,995,963 | 2025 | D20 | published | |
| SIERRA CLUB | Oakland, CA | $83,752,990 | 2025 | C30 | inferred 0.80 | |
| WOODLAND PARK ZOOLOGICAL SOCIETY | Seattle, WA | $83,715,805 | 2024 | D50 | published | science: research education |
| HOUSTON ZOO INC | Houston, TX | $82,689,282 | 2024 | D11 | published | space: outer |
| OMAHA ZOOLOGICAL SOCIETY INC | Omaha, NE | $82,044,812 | 2024 | D50 | published | science: research education |
| GREAT LAKES FISHERY COMMISSION | Ann Arbor, MI | $80,320,800 | 2024 | D33 | published | |
| RESOURCES LEGACY FUND | Sacramento, CA | $79,596,936 | 2024 | C30 | published | shelter: disaster |
| CLIMATE POWER | Washington, DC | $79,404,156 | 2024 | C20 | published | survivor: disaster |
| PAINTCARE INC | Washington, DC | $77,136,896 | 2025 | C27 | published | |
| ATLANTA BOTANICAL GARDEN INC | Atlanta, GA | $76,001,816 | 2024 | C41 | published | |
| LEAGUE OF CONSERVATION VOTERS EDUCATION FUND | Washington, DC | $75,210,318 | 2024 | C01 | published | |
| REWILD | Austin, TX | $75,177,871 | 2024 | D30 | published | science: research education |
| C40 CITIES CLIMATE LEADERSHIP GROUP INC | New York, NY | $73,614,205 | 2024 | C02 | published | shelter: homeless |
| OPEN SPACE INSTITUTE LAND TRUST INC | New York, NY | $73,114,837 | 2024 | C34 | published | girls boys: girl scouts |
| PETSMART CHARITIES INC | Phoenix, AZ | $71,189,403 | 2025 | D12 | published | shelter: animal |
| NOKUSE EDUCATION INC | Freeport, FL | $70,910,969 | 2025 | C30 | published | |
| NASHVILLE ZOO INC | Nashville, TN | $69,968,685 | 2025 | D50 | published | science: research education |
| ZOOLOGICAL SOCIETY OF CINCINNATI | Cincinnati, OH | $69,378,528 | 2024 | D50 | published | |
| ALLIANCE TO END PLASTIC WASTE INC | Austin, TX | $68,296,509 | 2024 | C01 | published | shelter: disaster |
| CHICAGO HORTICULTURAL SOCIETY | Glencoe, IL | $68,147,549 | 2024 | C41 | published | science: research education |
| SAVE THE REDWOODS LEAGUE | San Francisco, CA | $68,106,119 | 2024 | C36 | published | |
| SAN DIEGO HUMANE SOCIETY AND SPCA | San Diego, CA | $67,557,221 | 2024 | D20 | published | domestic: animals |
| THE NATIONAL TRUST FOR SCOTLAND | | $66,982,945 | 2013 | C30 | inferred 0.60 | |
| PALLADIUM FOUNDATION | Salt Lake Cty, UT | $66,882,185 | 2024 | C34 | published | |
| ALLIED CLIMATE PARTNERS INC | New York, NY | $66,539,772 | 2024 | C02 | published | |
| DENVER ZOOLOGICAL FOUNDATION INC | Denver, CO | $66,039,623 | 2025 | D11 | published | domestic: animals |
| MULTIPLIER | San Francisco, CA | $65,807,518 | 2024 | C60 | published | women: issues |
| DUCKS UNLIMITED INC | Memphis, TN | $65,801,593 | 2024 | C30 | published | |
| GOVERNORS ISLAND CORPORATION | New York, NY | $65,325,995 | 2024 | D30 | published | |
| DETROIT ZOOLOGICAL SOCIETY | Royal Oak, MI | $64,149,877 | 2024 | D50 | published | abuse: substance |
| ARIZONA CENTER FOR NATURE CONSERVATION | Phoenix, AZ | $63,967,380 | 2024 | D50 | published | |
| PENINSULA OPEN SPACE TRUST | Palo Alto, CA | $62,253,306 | 2024 | C50 | published | launch: launch |
| FORT WORTH ZOOLOGICAL ASSOCIATION INC | Fort Worth, TX | $62,181,894 | 2024 | D50 | published | |
| AUDUBON NATURE INSTITUTE INC | New Orleans, LA | $61,293,521 | 2024 | D12 | published | domestic: animals |
| SUNRISE PROJECT INC | Wilmington, DE | $61,111,981 | 2024 | C60 | published | |
| SAN ANTONIO ZOOLOGICAL SOCIETY INC | San Antonio, TX | $61,090,159 | 2024 | D50 | published | |
| MISSOURI BOTANICAL GARDEN BOARD OF TRUSTEES | Saint Louis, MO | $60,959,371 | 2024 | C41 | published | science: research education |
| ELEVATE ENERGY | Chicago, IL | $60,915,201 | 2024 | C35 | published | |
| HUDSON HIGHLANDS FJORD TRAIL INC | Poughkeepsie, NY | $60,054,466 | 2024 | C11 | published | |
| AQUARIUM OF THE PACIFIC | Long Beach, CA | $59,309,760 | 2024 | D50 | published | science: research education |
| SAN FRANCISCO SOCIETY FOR THE PREVENTION OF CRUELTY TO ANIMALS | San Francisco, CA | $58,331,987 | 2024 | D20 | published | shelter: animal |
| SOUTHERN ENVIRONMENTAL LAW CENTER | Charlottesville, VA | $58,167,590 | 2024 | C01 | inferred 0.85 | |
| TRUSTEES OF RESERVATIONS | Boston, MA | $57,850,047 | 2024 | C34 | published | girls boys: boys girls clubs |
| MORTON ARBORETUM | Lisle, IL | $57,793,716 | 2024 | C41 | published | science: research education |
| INDIANAPOLIS ZOOLOGICAL SOCIETY INC | Indianapolis, IN | $57,679,714 | 2024 | D50 | published | |