| ROCKY MOUNTAIN INSTITUTE | $174,917,694 | C34 | inferred 0.60 | | Boulder, CO | 2024 |
| URBAN LAND INSTITUTE | $92,328,399 | C34 | published | women: issues | Washington, DC | 2024 |
| OPEN SPACE INSTITUTE LAND TRUST INC | $73,114,837 | C34 | published | girls boys: girl scouts | New York, NY | 2024 |
| PALLADIUM FOUNDATION | $66,882,185 | C34 | published | | Salt Lake Cty, UT | 2024 |
| TRUSTEES OF RESERVATIONS | $57,850,047 | C34 | published | girls boys: boys girls clubs | Boston, MA | 2024 |
| REDWOOD FOREST FOUNDATION INC | $53,643,874 | C34 | published | science: research education | Fort Bragg, CA | 2024 |
| COLORADO OPEN LANDS | $51,292,712 | C34 | published | | Lakewood, CO | 2024 |
| THE LIVING DESERT | $47,987,318 | C34 | published | | Palm Desert, CA | 2024 |
| PRAIRIE STATE CONSERVATION COALITION | $47,481,503 | C34 | inferred 0.70 | science: research education | Springfield, IL | 2024 |
| MDS FAMLY FOUNDATION INC | $45,846,490 | C34 | published | | Maitland, FL | 2025 |
| AMERICAN PRAIRIE FOUNDATION | $44,696,566 | C34 | published | | Bozeman, MT | 2024 |
| LAND TRUST OF SANTA CRUZ COUNTY | $43,880,016 | C34 | published | | Santa Cruz, CA | 2024 |
| WESTERN PENNSYLVANIA CONSERVANCY | $41,640,928 | C34 | published | science: research education | Pittsburgh, PA | 2025 |
| NAPA COUNTY LAND TRUST | $39,723,786 | C34 | published | | Napa, CA | 2024 |
| WILDERNESS SOCIETY | $38,258,071 | C34 | published | science: research education | Washington, DC | 2024 |
| CHESAPEAKE BAY TRUST | $36,042,524 | C34 | inferred 0.60 | | Annapolis, MD | 2024 |
| GRAND TRAVERSE REGIONAL LAND CONSERVANCY | $34,487,626 | C34 | published | | Traverse City, MI | 2024 |
| WESTERN RESERVE LAND CONSERVANCY | $32,032,869 | C34 | published | | Moreland Hls, OH | 2024 |
| SOUTHERN APPALACHIAN HIGHLANDS CONSERVANCY | $30,646,831 | C34 | published | science: research education | Asheville, NC | 2024 |
| PLACER LAND TRUST | $30,050,893 | C34 | published | | Auburn, CA | 2024 |
| CENTER FOR NATURAL LANDS MANAGEMENT | $28,627,614 | C34 | published | science: research education | Temecula, CA | 2024 |
| PECONIC LAND TRUST INCORPORATED | $28,088,835 | C34 | published | | Southampton, NY | 2024 |
| TEXAS AGRICULTURAL LAND TRUST | $27,757,205 | C34 | published | | San Antonio, TX | 2024 |
| VOLGENAU FOUNDATION | $27,166,487 | C34 | published | science: research education | Mclean, VA | 2024 |
| NATURAL LANDS TRUST INCORPORATED | $26,592,726 | C34 | published | science: research education | Media, PA | 2024 |
| MONTANA LAND RELIANCE | $26,137,405 | C34 | published | | Helena, MT | 2024 |
| TRIANGLE LAND CONSERVANCY INC | $26,081,648 | C34 | published | girls boys: girl scouts | Durham, NC | 2024 |
| MINNESOTA LAND TRUST | $25,825,253 | C34 | published | | Saint Paul, MN | 2024 |
| MAINE COAST HERITAGE TRUST | $21,977,989 | C34 | published | | Topsham, ME | 2024 |
| CONSERVING CAROLINA | $21,581,120 | C34 | published | science: research education | Hendersonvlle, NC | 2024 |
| REALTY GIFT FUND | $20,802,312 | C34 | published | | Santa Fe, NM | 2024 |
| VERMONT LAND TRUST INC | $20,570,203 | C34 | published | | Montpelier, VT | 2024 |
| BIG SUR LAND TRUST | $17,754,257 | C34 | published | | Monterey, CA | 2024 |
| SCONSET TRUST INC | $16,273,561 | C34 | published | | Siasconset, MA | 2024 |
| ER NERR LAND FUND | $15,787,982 | C34 | published | | Klamath, CA | 2024 |
| LAND LEGACY | $14,118,114 | C34 | published | | Tulsa, OK | 2025 |
| WILDLANDS CONSERVANCY | $13,845,567 | C34 | published | science: research education | Yucaipa, CA | 2024 |
| COLUMBIA LAND TRUST | $13,793,237 | C34 | published | | Vancouver, WA | 2024 |
| NOVA SCOTIA NATURE TRUST | $13,181,388 | C34 | published | | Canada | 2024 |
| THE LAND TRUST FOR SANTA BARBARA COUNTY | $12,961,913 | C34 | published | | Santa Barbara, CA | 2024 |
| CALIFORNIA FARMLAND TRUST | $12,813,821 | C34 | published | | Elk Grove, CA | 2024 |
| FORTERRA NW | $12,806,098 | C34 | published | science: research education | Seattle, WA | 2024 |
| FRIENDS OF ACADIA | $12,663,310 | C34 | published | science: research education | Bar Harbor, ME | 2025 |
| LOWCOUNTRY LAND TRUST INC | $12,627,339 | C34 | published | | Charleston, SC | 2024 |
| SEMPERVIRENS FUND | $12,609,954 | C34 | published | | Mountain View, CA | 2024 |
| SOGOREA TE LAND TRUST | $12,536,415 | C34 | published | | Oakland, CA | 2024 |
| MESA COUNTY LAND CONSERVANCY INC | $12,402,360 | C34 | published | | Grand Jct, CO | 2024 |
| HIIPAKA LLC | $12,384,559 | C34 | published | | Haleiwa, HI | 2025 |
| SCENIC HUDSON LAND TRUST INC | $12,214,477 | C34 | published | science: research education | Poughkeepsie, NY | 2024 |
| THE CALIFORNIA DESERT LAND CONSERVANCY | $12,174,899 | C34 | published | science: research education | Joshua Tree, CA | 2024 |
| PIEDMONT ENVIRONMENTAL COUNCIL | $12,005,795 | C34 | published | | Warrenton, VA | 2024 |
| CONSERVATION FLORIDA INC | $11,503,770 | C34 | published | science: research education | Orlando, FL | 2025 |
| UTAH OPEN LANDS CONSERVATION ASSOCIATION INC | $11,369,279 | C34 | published | | Salt Lake Cty, UT | 2024 |
| NORTH FLORIDA LAND TRUST INC | $11,248,165 | C34 | published | | Jacksonville, FL | 2024 |
| LITTLE TRAVERSE CONSERVANCY INC | $11,225,910 | C34 | published | | Harbor Spgs, MI | 2024 |
| GEORGIA-ALABAMA LAND TRUST INC | $11,187,062 | C34 | published | | Piedmont, AL | 2024 |
| NORTH DAKOTA NATURAL RESOURCES TRUST INC | $11,068,125 | C34 | inferred 0.60 | science: research education | Bismarck, ND | 2025 |
| GREAT PENINSULA CONSERVANCY | $10,980,530 | C34 | published | | Bremerton, WA | 2024 |
| TRUCKEE DONNER LAND TRUST | $10,482,710 | C34 | published | science: research education | Truckee, CA | 2024 |
| EDWARD E HADDDOCK JR FAMILY OPERATING FOUNDATION | $10,261,095 | C34 | published | women: issues | Orlando, FL | 2024 |
| VITAL GROUND FOUNDATION INC | $10,218,738 | C34 | inferred 0.85 | domestic: animals | Missoula, MT | 2025 |
| UNITED STATES ENDOWMENT FORFORESTRY AND COMMUNITIES INC | $10,178,279 | C34 | published | science: research education | Greenville, SC | 2024 |
| CONSERVATION LANDS FOUNDATION | $10,166,862 | C34 | published | | Durango, CO | 2025 |
| PIEDMONT LAND CONSERVANCY | $10,164,468 | C34 | published | | Greensboro, NC | 2025 |
| THE FINGER LAKES LAND TRUST INC | $10,034,901 | C34 | published | | Ithaca, NY | 2024 |
| JACKSON HOLE LAND TRUST | $9,828,394 | C34 | published | | Jackson, WY | 2024 |
| NORTHWEST CONNECTICUT LAND CONSERVANCY INC | $9,554,105 | C34 | published | | Kent, CT | 2024 |
| LANCASTER FARMLAND TRUST | $9,486,707 | C34 | published | | Strasburg, PA | 2025 |
| FLATHEAD LAND TRUST INC | $9,415,179 | C34 | published | | Kalispell, MT | 2024 |
| LAKE FOREST OPEN LANDS ASSOCIATION | $9,393,257 | C34 | published | science: research education | Mettawa, IL | 2025 |
| ALACHUA CONSERVATION TRUST INCORPORATED | $9,376,838 | C34 | published | men: issues | Gainesville, FL | 2024 |
| ORGANIC MATERIALS REVIEW INSTITUTE | $9,095,107 | C34 | published | science: research education | Eugene, OR | 2024 |
| ESSEX COUNTY GREENBELT ASSOCIATION | $8,983,643 | C34 | published | | Essex, MA | 2024 |
| PRICKLY PEAR LAND TRUST | $8,891,943 | C34 | published | | Helena, MT | 2024 |
| ORLEANS CONSERVATION TRUST | $8,857,346 | C34 | published | | Orleans, MA | 2024 |
| JOHN MUIR LAND TRUST | $8,835,839 | C34 | published | | Martinez, CA | 2024 |
| NATURALAND TRUST | $8,682,962 | C34 | published | girls boys: boy scouts | Greenville, SC | 2024 |
| LAND CONSERVATION TRUST | $8,667,779 | C34 | inferred 0.80 | | Chicago, IL | 2020 |
| WHIDBEY-CAMANO LAND TRUST | $8,631,910 | C34 | published | | Greenbank, WA | 2025 |
| ROAD HOME CORPORATION | $8,057,780 | C34 | published | | Baton Rouge, LA | 2024 |
| FOOTHILLS CONSERVANCY OF NORTH CAROLINA INC | $8,056,851 | C34 | published | | Morganton, NC | 2025 |
| MCKENZIE RIVER TRUST | $7,947,444 | C34 | published | | Eugene, OR | 2024 |
| WILDLIFE HERITAGE FOUNDATION | $7,931,115 | C34 | published | | Lincoln, CA | 2024 |
| LAKE GEORGE LAND CONSERVANCY INC | $7,750,481 | C34 | published | | Bolton Lndg, NY | 2024 |
| APPALACHIA OHIO ALLIANCE | $7,713,657 | C34 | published | science: research education | Columbus, OH | 2024 |
| CONSERVATION FOUNDATION | $7,541,114 | C34 | published | girls boys: boys girls clubs | Naperville, IL | 2024 |
| UNCOMPAHGRE VALLEY WATER USERS | $7,486,462 | C34 | inferred 0.45 | | Montrose, CO | 2024 |
| HILL COUNTRY CONSERVANCY INC | $7,443,901 | C34 | published | | Austin, TX | 2025 |
| WOOD RIVER LAND TRUST COMPANY | $7,307,675 | C34 | published | | Hailey, ID | 2024 |
| CATAWBA LANDS CONSERVANCY | $7,293,034 | C34 | published | girls boys: girl scouts | Charlotte, NC | 2024 |
| FRIENDS OF SANTA CRUZ STATE PARKS | $7,259,454 | C34 | inferred 0.50 | | Santa Cruz, CA | 2024 |
| THE LAND CONSERVANCY OF SAN LUIS OBISPO COUNTY | $7,223,040 | C34 | published | | Sn Luis Obisp, CA | 2024 |
| CENTRAL INDIANA LAND TRUST INCORPORATED | $7,176,209 | C34 | published | | Indianapolis, IN | 2024 |
| ALLEGHENY LAND TR | $6,972,178 | C34 | published | | Sewickley, PA | 2024 |
| LEGACY LAND CONSERVANCY | $6,942,265 | C34 | published | | Ann Arbor, MI | 2024 |
| THE MONADNOCK CONSERVANCY | $6,871,141 | C34 | published | girls boys: girl scouts | Keene, NH | 2024 |
| ACRES INC | $6,857,014 | C34 | published | science: research education | Huntertown, IN | 2024 |
| MAINSPRING CONSERVATION TRUST INC | $6,684,058 | C34 | published | science: research education | Franklin, NC | 2024 |
| BLUEGRASS LAND CONSERVANCY INC | $6,616,412 | C34 | published | | Lexington, KY | 2024 |
| LANDPATHS | $6,581,248 | C34 | published | violence: community | Santa Rosa, CA | 2024 |