| THE ANIMAL MEDICAL CENTER | $96,735,746 | D40 | published | domestic: animals | New York, NY | 2024 |
| DOVE LEWIS EMERGENCY ANIMAL HOSPITAL INC | $35,964,307 | D40 | published | domestic: animals | Portland, OR | 2024 |
| COMMUNITY ANIMAL MEDICINE PROJECT | $11,494,574 | D40 | published | shelter: animal | San Pedro, CA | 2024 |
| PAWS OF HONOR INC | $10,592,256 | D40 | published | | Williamsburg, VA | 2024 |
| CHRISTIAN VETERINARY MISSION FOUNDATION | $9,138,410 | D40 | published | launch: religious | Lynnwood, WA | 2024 |
| ROCHESTER EMERGENCY VETERINARY SERVICES | $7,510,528 | D40 | published | | East Rochester, NY | 2024 |
| PETS UNLIMITED | $5,452,371 | D40 | inferred 0.85 | shelter: animal | San Francisco, CA | 2013 |
| NINE LIVES FOUNDATION | $5,134,031 | D40 | published | shelter: animal | Redwood City, CA | 2025 |
| BRIDGE CLINIC INC | $4,371,090 | D40 | published | | Bensalem, PA | 2024 |
| ANICIRA VETERINARY CENTER - CALIFORNIA INC | $3,394,063 | D40 | published | shelter: animal | Harrisonburg, VA | 2025 |
| LOW COST ANIMAL MEDICAL CENTER | $3,333,545 | D40 | published | | New Orleans, LA | 2024 |
| ALTERCARE ANIMAL CLINIC | $2,948,619 | D40 | published | | Canton, OH | 2025 |
| SAM SIMON CHARITABLE FOUNDATION | $2,801,712 | D40 | inferred 0.60 | | Los Angeles, CA | 2024 |
| HARD LUCK ANIMAL WELFARE ADVOCATES | $2,694,828 | D40 | published | shelter: animal | Sacramento, CA | 2025 |
| PET COMMUNITY CENTER INC | $2,623,553 | D40 | published | shelter: animal | Nashville, TN | 2025 |
| SPEAK ANIMAL HOSPITAL PC | $2,616,588 | D40 | published | | Binghamton, NY | 2025 |
| PLANNED PETHOOD INTERNATIONAL | $2,463,647 | D40 | published | shelter: animal | Conifer, CO | 2025 |
| HUMANE ALLIANCE OF WNC INC | $2,450,417 | D40 | inferred 0.55 | | Asheville, NC | 2015 |
| HEMOPET | $2,408,136 | D40 | published | science: research education | Garden Grove, CA | 2024 |
| ANIMAL TRUSTEES OF AUSTIN INC | $2,292,732 | D40 | inferred 0.90 | abuse: animal | Austin, TX | 2015 |
| AMERICAN ASSOCIATION OF ZOO VETERINARIANS | $2,018,669 | D40 | published | science: research education | Jacksonville, FL | 2023 |
| THE PATTERSON FOUNDATION | $1,979,390 | D40 | published | | Flowery Br, GA | 2025 |
| BROWNSVILLE SPAY NEUTER INC | $1,924,655 | D40 | published | shelter: animal | Olmito, TX | 2024 |
| PROJECT STREET VET | $1,897,754 | D40 | published | | Albuquerque, NM | 2025 |
| BLOOMINGTON PETS ALIVE INC | $1,665,347 | D40 | published | shelter: animal | Bloomington, IN | 2024 |
| CHASE ANIMAL RESCUE AND SANCTUARY INC | $1,619,825 | D40 | published | domestic: animals | Webster, FL | 2025 |
| PUBLIC VET | $1,610,137 | D40 | published | | Bloomington, IN | 2025 |
| HUMANE SOCIETY AC VETERINARY SERVICES PC | $1,594,595 | D40 | published | | Atlantic City, NJ | 2024 |
| Vet Care Coalition | $1,587,402 | D40 | inferred 0.50 | | Denver, CO | 2024 |
| SAVING HOPE VETERINARY CLINIC | $1,559,078 | D40 | published | | Fort Worth, TX | 2024 |
| FOR THE LOVE OF PAWS | $1,536,716 | D40 | inferred 0.85 | | Roseburg, OR | 2025 |
| WEST SOUND WILDLIFE SHELTER | $1,471,093 | D40 | published | shelter: animal | Bainbridge Is, WA | 2025 |
| NEBRASKA WILDLIFE REHAB INC | $1,453,325 | D40 | published | science: research education | Omaha, NE | 2024 |
| VETS IN VANS INC | $1,406,301 | D40 | published | shelter: animal | Oakland, CA | 2024 |
| LAZARUS VETERINARY CLINICS INC | $1,371,524 | D40 | inferred 0.80 | shelter: animal | Deforest, WI | 2022 |
| LOVE PET PROJECT INC | $1,325,881 | D40 | published | shelter: animal | Zionsville, IN | 2024 |
| CHEW ANIMAL CLINIC | $1,302,181 | D40 | published | shelter: animal | Dallas, TX | 2024 |
| SPAY NEUTER INCENTIVE PROGRAM OF WEST VIRGINIA | $1,242,982 | D40 | inferred 0.60 | | Morgantown, WV | 2024 |
| CRITTER CONNECTION | $1,231,458 | D40 | published | shelter: animal | Chapmanville, WV | 2025 |
| MAINE ANIMAL HEALTH ALLIANCE | $1,230,911 | D40 | published | | Augusta, ME | 2024 |
| MISFIT SPAY NEUTER CLINIC INC | $1,199,327 | D40 | published | shelter: animal | Tavares, FL | 2025 |
| SPAY NEUTER SAVE NETWORK | $1,179,083 | D40 | published | | Rome, PA | 2024 |
| PET CARE COALITION INC | $1,131,612 | D40 | published | | Aurora, CO | 2024 |
| SNIPWELL SPAY NEUTER & WELLNESS CLINIC INC | $1,035,816 | D40 | published | | Fort Mill, SC | 2025 |
| ROBINSONS RESCUE INC | $957,637 | D40 | published | | Shreveport, LA | 2024 |
| NOTHING BUT LUCK INC | $953,053 | D40 | published | | Kernersville, NC | 2023 |
| FOUR PRECIOUS PAWS LOW COST SPAY & NEUTER CLINIC INC | $933,083 | D40 | published | domestic: animals | Lafayette, IN | 2024 |
| STREET CAT CLINIC INC | $831,765 | D40 | published | | Miami, FL | 2025 |
| SEATTLE VETERINARY OUTREACH | $820,359 | D40 | inferred 0.70 | | Seattle, WA | 2025 |
| DEVELOPING VETERINARY MEDICINE CAREERS | $817,575 | D40 | published | | Vancouver, WA | 2024 |
| SOUTHWEST FLORIDA SPAY NEUTER SERVICES INC | $805,237 | D40 | published | shelter: animal | Englewood, FL | 2025 |
| ARCHDALE AFFORDABLE NEUTER & WELLNESS CLINIC INC | $779,750 | D40 | published | shelter: animal | Archdale, NC | 2024 |
| OCONNOR FOUNDATION | $743,565 | D40 | published | | Willow Spgs, IL | 2024 |
| FRANKIES FRIENDS INC | $743,028 | D40 | published | | Tampa, FL | 2024 |
| DOG AIDE | $741,210 | D40 | published | abuse: animal | Detroit, MI | 2024 |
| RILEYS URGENT FUND FOR FRIENDS | $710,907 | D40 | published | | Gallatin Gateway, MT | 2025 |
| INDIANA VETERINARY MEDICAL ASSOCIATION | $707,580 | D40 | inferred 0.85 | science: research education | Indianapolis, IN | 2024 |
| BE THE SOLUTION INC | $706,168 | D40 | published | | Tallahassee, FL | 2024 |
| CATSNIP CARES FOUNDATION | $643,832 | D40 | published | | Snellville, GA | 2025 |
| ENCOMPASS ANIMAL HEALTH INC | $624,072 | D40 | published | | Hamburg, NY | 2025 |
| VIN FOUNDATION | $617,653 | D40 | published | science: research education | Davis, CA | 2024 |
| LEGACY HUMANE VETERINARY CLINIC | $576,186 | D40 | published | | Mckinney, TX | 2025 |
| RESCUED PETS ARE WONDERFUL ANIMAL WELLNESS CENTER PC | $566,697 | D40 | published | shelter: animal | Forest Lake, MN | 2025 |
| DELANEY WYKLE ANIMAL WELLNESS CENTER INC | $565,322 | D40 | published | | Ghent, WV | 2024 |
| UNITED HUMANITARIANS A CORPORATION | $557,605 | D40 | inferred 0.70 | shelter: animal | Cocoa, FL | 2018 |
| KAT VET SPAY NEUTER & RESCUE | $542,661 | D40 | inferred 0.85 | | Ponchatoula, LA | 2024 |
| DONEY MEMORIAL PET CLINIC | $530,175 | D40 | published | | Seattle, WA | 2024 |
| JUST FOUR PAWS FOUNDATION | $528,330 | D40 | published | shelter: animal | Apple Valley, CA | 2025 |
| FRIENDS RESCUED ANIMALS NETWORK | $525,312 | D40 | published | | Sisters, OR | 2024 |
| ANGEL OF HOPE ANIMAL RESCUE | $520,320 | D40 | published | | Dayton, MN | 2024 |
| ANIMAL RESCUE VETERINARY SERVICES | $501,926 | D40 | published | shelter: animal | Londonderry, NH | 2025 |
| HEART HEALTHCARE & EMERGENCY ANIMAL RESCUE TEAM | $481,796 | D40 | published | shelter: animal | Yorba Linda, CA | 2024 |
| RESCUE CITY INC | $472,465 | D40 | published | abuse: animal | Brooklyn, NY | 2025 |
| THE NATIONAL ORGANIZATION TO END PET OVERPOPULATION INC | $461,107 | D40 | inferred 0.85 | | Lawton, OK | 2025 |
| VETERINARY COMMUNITY OUTREACH INC | $458,275 | D40 | published | | Berryville, VA | 2025 |
| FOX VALLEY VETERANS COUNCIL INCORPORATED | $451,877 | D40 | published | | Appleton, WI | 2024 |
| Boston's Pet Haven Inc | $450,146 | D40 | inferred 0.80 | shelter: animal | Watertown, MA | 2013 |
| LAURELS HOUSE | $437,666 | D40 | inferred 0.80 | shelter: animal | Houston, TX | 2021 |
| EL PASO VETERINARY MEDICAL ASSOCIATION | $436,524 | D40 | inferred 0.60 | | El Paso, TX | 2025 |
| WE CARE LOW COST SPAY NEUTER CLINIC INC | $434,498 | D40 | published | | Jesup, GA | 2025 |
| BEND SPAY & NEUTER PROJECT | $430,095 | D40 | inferred 0.85 | shelter: animal | Bend, OR | 2018 |
| VFW DEPARTMENT OF CALIFORNIA FOUNDATION | $424,430 | D40 | published | | Elk Grove, CA | 2024 |
| SUWANNEE PAWS INC | $418,447 | D40 | published | shelter: animal | O Brien, FL | 2025 |
| LUNA VET HELP | $416,296 | D40 | published | | San Juan, PR | 2025 |
| VETERINARIANS FOR PUERTO RICO CORP | $413,780 | D40 | published | | Columbia, MO | 2025 |
| GREAT DANE CLUB OF AMERICA CHARITABLE TR | $404,926 | D40 | published | science: research education | Kenner, LA | 2024 |
| PIPER FOUNDATION | $400,000 | D40 | published | | Bloomfld Hls, MI | 2024 |
| TEXAS FOUNDATION FOR ANIMAL CARE | $398,958 | D40 | published | | Ft Worth, TX | 2025 |
| SPAY NEUTER INTERVENTION OF THE PEE DEE | $392,318 | D40 | inferred 0.90 | shelter: animal | Florence, SC | 2021 |
| WORLDWIDE VETS INCORPORATED | $379,676 | D40 | published | domestic: animals | Lees Summit, MO | 2024 |
| HELPING EVERY ANIMAL RECEIVE TREATMENT | $378,337 | D40 | published | shelter: animal | Annapolis, MD | 2025 |
| ELLIES RAINY DAY FUND INC | $376,802 | D40 | published | domestic violence | Beavercreek, OH | 2025 |
| AMERICAN VETERINARY CHIROPRACTIC | $371,802 | D40 | inferred 0.60 | | Bluejacket, OK | 2024 |
| AMERICAN COLLEGE OF VETERINARY PREVENTIVE MEDICINE | $368,504 | D40 | published | | Richland, MI | 2025 |
| LIFE SOURCE TRAINING INSTITUTE INC | $366,890 | D40 | published | | Riverside, CA | 2024 |
| RI Companion Animal Alliance | $356,079 | D40 | inferred 0.85 | | Riverside, RI | 2020 |
| COMMUNITY VETERINARY CENTER | $354,244 | D40 | inferred 0.80 | shelter: animal | Pleasant Hill, OR | 2024 |
| CROSSROADS VETERINARY CLINIC PC | $351,762 | D40 | published | | Buffalo, MN | 2024 |
| FLORIDA VETERINARY MEDICAL ASSOCIATION FOUNDATION INC | $328,616 | D40 | published | | Orlando, FL | 2024 |
| SEQUATCHIE VALLEY LOW COST SPAY & NEUTER | $323,172 | D40 | published | shelter: animal | Dunlap, TN | 2024 |