| SAN DIEGO ZOO WILDLIFE ALLIANCE | $437,607,695 | D50 | published | science: research education | San Diego, CA | 2024 |
| WILDLIFE CONSERVATION SOCIETY | $400,861,377 | D50 | published | domestic: animals | Bronx, NY | 2024 |
| GEORGIA AQUARIUM INC | $165,667,302 | D50 | published | science: research education | Atlanta, GA | 2024 |
| SHEDD AQUARIUM SOCIETY | $101,974,802 | D50 | published | science: research education | Chicago, IL | 2024 |
| CHICAGO ZOOLOGICAL SOCIETY | $90,541,500 | D50 | published | science: research education | Brookfield, IL | 2025 |
| COLUMBUS ZOOLOGICAL PARK ASSOCIATION | $88,819,571 | D50 | published | | Powell, OH | 2024 |
| WOODLAND PARK ZOOLOGICAL SOCIETY | $83,715,805 | D50 | published | science: research education | Seattle, WA | 2024 |
| OMAHA ZOOLOGICAL SOCIETY INC | $82,044,812 | D50 | published | science: research education | Omaha, NE | 2024 |
| NASHVILLE ZOO INC | $69,968,685 | D50 | published | science: research education | Nashville, TN | 2025 |
| ZOOLOGICAL SOCIETY OF CINCINNATI | $69,378,528 | D50 | published | | Cincinnati, OH | 2024 |
| DETROIT ZOOLOGICAL SOCIETY | $64,149,877 | D50 | published | abuse: substance | Royal Oak, MI | 2024 |
| ARIZONA CENTER FOR NATURE CONSERVATION | $63,967,380 | D50 | published | | Phoenix, AZ | 2024 |
| FORT WORTH ZOOLOGICAL ASSOCIATION INC | $62,181,894 | D50 | published | | Fort Worth, TX | 2024 |
| SAN ANTONIO ZOOLOGICAL SOCIETY INC | $61,090,159 | D50 | published | | San Antonio, TX | 2024 |
| AQUARIUM OF THE PACIFIC | $59,309,760 | D50 | published | science: research education | Long Beach, CA | 2024 |
| INDIANAPOLIS ZOOLOGICAL SOCIETY INC | $57,679,714 | D50 | published | | Indianapolis, IN | 2024 |
| NEW ENGLAND AQUARIUM CORPORATION | $54,645,914 | D50 | published | space: outer | Boston, MA | 2024 |
| NATIONAL AQUARIUM INC | $54,351,794 | D50 | published | science: research education | Baltimore, MD | 2025 |
| JACKSONVILLE ZOOLOGICAL SOCIETY INC | $50,606,141 | D50 | published | | Jacksonville, FL | 2024 |
| LINCOLN PARK ZOOLOGICAL SOCIETY | $50,519,973 | D50 | published | science: research education | Chicago, IL | 2024 |
| TOLEDO ZOOLOGICAL SOCIETY | $50,015,283 | D50 | published | | Toledo, OH | 2024 |
| LOWRY PARK ZOOLOGICAL SOCIETY OF TAMPA INC | $48,990,299 | D50 | published | survivor: cancer | Tampa, FL | 2024 |
| ZOOLOGICAL SOCIETY OF PHILADELPHIA | $43,711,192 | D50 | published | domestic: animals | Philadelphia, PA | 2024 |
| ATLANTA-FULTON COUNTY ZOO INC | $42,311,626 | D50 | published | domestic: animals | Atlanta, GA | 2025 |
| FLORIDA AQUARIUM INC | $37,200,637 | D50 | published | girls boys: girls | Tampa, FL | 2024 |
| CONSERVATION SOCIETY OF CALIFORNIA | $35,008,424 | D50 | published | domestic: animals | Oakland, CA | 2024 |
| CHEYENNE MOUNTAIN ZOOLOGICAL SOCIETY | $34,386,132 | D50 | published | | Colorado Spgs, CO | 2025 |
| COMMONWEALTH ZOOLOGICAL CORPORATION | $34,370,304 | D50 | published | science: research education | Boston, MA | 2024 |
| TENNESSEE AQUARIUM | $34,216,548 | D50 | published | science: research education | Chattanooga, TN | 2024 |
| SEA RESEARCH FOUNDATION INC | $34,034,517 | D50 | published | space: outer | Mystic, CT | 2025 |
| EAST COAST ZOOLOGICAL SOCIETY OF FLORIDA INC | $30,844,454 | D50 | published | | Melbourne, FL | 2024 |
| MEMPHIS ZOO INC | $30,584,120 | D50 | published | | Memphis, TN | 2024 |
| ZOOLOGICAL SOCIETY OF PITTSBURGH | $30,459,605 | D50 | published | science: research education | Pittsburgh, PA | 2024 |
| FRESNOS CHAFFEE ZOO CORPORATION | $29,607,528 | D50 | published | | Fresno, CA | 2024 |
| ZOOLOGICAL SOCIETY OF THE PALM BEACHES INC | $26,669,019 | D50 | published | science: research education | West Palm Bch, FL | 2024 |
| AKRON ZOOLOGICAL PARK | $25,160,371 | D50 | published | domestic: animals | Akron, OH | 2024 |
| THE MARYLAND ZOOLOGICAL SOCIETY INC | $24,960,748 | D50 | published | | Baltimore, MD | 2024 |
| UTAH ZOOLOGICAL SOCIETY | $23,946,177 | D50 | published | science: research education | Salt Lake Cty, UT | 2025 |
| GULF OF MAINE RESEARCH INSTITUTE | $23,325,937 | D50 | published | space: outer | Portland, ME | 2024 |
| NORRISTOWN ZOOLOGICAL SOCIETY | $23,162,878 | D50 | published | | Norristown, PA | 2025 |
| SAN FRANCISCO ZOOLOGICAL SOCIETY | $23,127,512 | D50 | published | | San Francisco, CA | 2024 |
| SEDGWICK COUNTY ZOOLOGICAL SOCIETY INC | $22,916,066 | D50 | published | | Wichita, KS | 2024 |
| CLEARWATER MARINE AQUARIUM INC | $22,862,676 | D50 | published | science: research education | Clearwater, FL | 2024 |
| SANTA BARBARA ZOOLOGICAL FOUNDATION | $21,787,514 | D50 | published | | Santa Barbara, CA | 2024 |
| SOUTH CAROLINA AQUARIUM | $20,285,821 | D50 | published | science: research education | Charleston, SC | 2024 |
| TEXAS STATE AQUARIUM ASSOCIATION | $19,116,039 | D50 | published | science: research education | Corp Christi, TX | 2024 |
| OREGON COAST AQUARIUM INC | $18,336,343 | D50 | published | science: research education | Newport, OR | 2024 |
| KNOXVILLE ZOOLOGICAL GARDENS INC | $16,513,839 | D50 | published | | Knoxville, TN | 2024 |
| SIOUX FALLS ZOO & AQUARIUM | $16,102,971 | D50 | published | | Sioux Falls, SD | 2025 |
| NORTH CAROLINA ZOOLOGICAL SOCIETY INC | $15,456,610 | D50 | published | science: research education | Asheboro, NC | 2025 |
| CLEVELAND ZOOLOGICAL SOCIETY | $14,559,194 | D50 | published | science: research education | Cleveland, OH | 2024 |
| BIRMINGHAM ZOO INC | $14,419,714 | D50 | published | | Mountain Brk, AL | 2024 |
| RHODE ISLAND ZOOLOGICAL SOCIETY | $13,650,518 | D50 | published | | Providence, RI | 2024 |
| ZOO MIDLAND INC | $13,449,918 | D50 | published | | Midland, TX | 2024 |
| NAPLES ZOO INC | $13,360,275 | D50 | published | science: research education | Naples, FL | 2024 |
| ZOOLOGICAL SOCIETY OF BUFFALO INC | $12,655,840 | D50 | published | science: research education | Buffalo, NY | 2025 |
| SACRAMENTO ZOOLOGICAL SOCIETY | $12,366,549 | D50 | published | | Sacramento, CA | 2024 |
| LINCOLN CHILDRENS ZOO | $9,996,076 | D50 | published | | Lincoln, NE | 2024 |
| POTAWATOMI ZOOLOGICAL SOCIETY INC | $9,499,145 | D50 | published | | South Bend, IN | 2025 |
| VALLEY ZOOLOGICAL SOCIETY CHARTERED MAY 1968 | $9,450,573 | D50 | published | | Brownsville, TX | 2024 |
| ABILENE ZOOLOGICAL SOCIETY | $9,260,792 | D50 | published | | Abilene, TX | 2024 |
| CENTRAL FLORIDA ZOOLOGICAL SOCIETY INC | $8,989,141 | D50 | published | science: research education | Lake Monroe, FL | 2024 |
| ZOO MIAMI FOUNDATION INC | $7,879,169 | D50 | published | | Miami, FL | 2024 |
| MISSISSIPPI AQUARIUM | $7,657,771 | D50 | published | science: research education | Gulfport, MS | 2025 |
| BINDER PARK ZOOLOGICAL SOCIETY INC | $6,241,160 | D50 | published | | Battle Creek, MI | 2025 |
| STATEN ISLAND ZOOLOGICAL SOCIETY INC | $5,951,642 | D50 | published | | Staten Island, NY | 2024 |
| LEHIGH VALLEY ZOOLOGICAL SOCIETY | $5,859,349 | D50 | published | science: research education | Schnecksville, PA | 2024 |
| VIRGINIA ZOOLOGICAL SOCIETY | $5,335,550 | D50 | published | science: research education | Norfolk, VA | 2024 |
| CONNECTICUT ZOOLOGICAL SOCIETY INC | $5,274,501 | D50 | published | | Bridgeport, CT | 2024 |
| ERIE ZOOLOGICAL SOCIETY | $5,146,557 | D50 | published | science: research education | Erie, PA | 2024 |
| GREAT RIVER GREENING | $4,983,997 | D50 | published | science: research education | Saint Paul, MN | 2024 |
| ZOO FOUNDATION INC | $4,971,469 | D50 | published | | Gulf Shores, AL | 2024 |
| NIAGARA AQUARIUM FOUNDATION INC | $4,890,455 | D50 | published | science: research education | Niagara Falls, NY | 2024 |
| RACINE ZOOLOGICAL SOCIETY | $3,847,150 | D50 | published | domestic: animals | Racine, WI | 2024 |
| ALASKA ZOO | $3,469,188 | D50 | published | shelter: animal | Anchorage, AK | 2025 |
| PUEBLO ZOOLOGICAL SOCIETY | $2,946,096 | D50 | published | | Pueblo, CO | 2025 |
| SOUTHERN TIER ZOOLOGICAL SOCIETY INC | $2,943,896 | D50 | published | | Binghamton, NY | 2024 |
| LAKE SUPERIOR ZOOLOGICAL SOCIETY | $2,922,401 | D50 | published | launch: launch | Duluth, MN | 2024 |
| SAGINAW VALLEY ZOOLOGICAL SOCIETY | $2,798,522 | D50 | published | | Saginaw, MI | 2025 |
| GLOBAL WILDLIFE TRUST INC | $2,754,192 | D50 | published | | Thurmont, MD | 2024 |
| UTICA ZOOLOGICAL SOCIETY | $2,693,753 | D50 | published | science: research education | Utica, NY | 2024 |
| HEALTHY KIDS RUNNING SERIES | $2,653,079 | D50 | published | | Chadds Ford, PA | 2025 |
| DAKOTA ZOOLOGICAL SOCIETY INC | $2,587,633 | D50 | published | domestic: animals | Bismarck, ND | 2025 |
| FRIENDS OF THE ZOO OF SPRINGFIELD MISSOURI INC | $2,436,476 | D50 | published | | Springfield, MO | 2024 |
| BIG CAT HABITAT GULF COAST SANCTUARY INC | $2,352,226 | D50 | published | domestic: animals | Sarasota, FL | 2025 |
| ZOOMONTANA INC | $2,270,039 | D50 | published | | Billings, MT | 2024 |
| RED RIVER ZOOLOGICAL SOCIETY | $2,164,124 | D50 | published | | Fargo, ND | 2024 |
| BUTTONWOOD PARK ZOOLOGICAL SOCIETY INC | $1,988,083 | D50 | published | | New Bedford, MA | 2025 |
| TIGER PRESERVATION CENTER INC | $1,705,482 | D50 | inferred 0.60 | | Cave Junction, OR | 2019 |
| MONTEREY ZOOLOGICAL SOCIETY | $1,701,441 | D50 | published | | Salinas, CA | 2024 |
| ZOOLOGICAL SOCIETY OF WASHINGTON | $1,563,019 | D50 | published | | Issaquah, WA | 2024 |
| BLUE RIDGE ZOOLOGICAL SOCIETY OF VIRGINIA INC | $1,557,783 | D50 | published | | Roanoke, VA | 2024 |
| POTTER PARK ZOOLOGICAL SOCIETY | $1,499,981 | D50 | published | girls boys: girl scouts | Lansing, MI | 2025 |
| HAPPY HOLLOW CORPORATION | $1,431,641 | D50 | published | girls boys: girl scouts | San Jose, CA | 2024 |
| DELAWARE ZOOLOGICAL SOCIETY INC | $1,323,879 | D50 | published | | Wilmington, DE | 2025 |
| EVANSVILLE ZOOLOGICAL SOCIETY INC | $1,275,640 | D50 | published | | Evansville, IN | 2025 |
| FOREST PARK ZOOLOGICAL SOCIETY INC | $1,264,747 | D50 | published | | Springfield, MA | 2024 |
| AQUARIUM OF BOISE INC | $1,252,820 | D50 | published | | Boise, ID | 2024 |
| THE CENTER FOR AQUATIC SCIENCES INC | $1,215,781 | D50 | published | science: research education | Camden, NJ | 2024 |
| EAST IDAHO AQUARIUM CORPORATION | $1,202,548 | D50 | published | | Idaho Falls, ID | 2024 |