| THE WILD ANIMAL SANCTUARY A COLORADO NON-PROFIT CORPORATION | $38,304,328 | D34 | published | abuse: animal | Keenesburg, CO | 2025 |
| NEW ENGLAND WILDLIFE CENTER | $30,843,812 | D34 | published | | S Weymouth, MA | 2024 |
| OPERATION KINDNESS | $17,631,228 | D34 | published | shelter: animal | Carrollton, TX | 2024 |
| INTERNATIONAL CENTER FOR THE PRESERVATION OF WILD ANIMALS INC | $11,812,050 | D34 | published | | Powell, OH | 2024 |
| WILDLIFE ALLIANCE INC | $9,233,046 | D34 | published | domestic: animals | New York, NY | 2024 |
| BIG LIFE FOUNDATION USA | $7,890,389 | D34 | published | | Seattle, WA | 2025 |
| NEW MEXICO WILDLIFE CENTER INC | $6,945,151 | D34 | inferred 0.80 | science: research education | Espanola, NM | 2024 |
| CENTER FOR ORANGUTAN AND CHIMPANZEE CONSERVATION INC | $6,762,159 | D34 | published | | Wauchula, FL | 2024 |
| SOUTHWEST WILDLIFE CONSERVATION CENTER | $6,459,999 | D34 | published | science: research education | Scottsdale, AZ | 2025 |
| NATIONAL DEER ASSOCIATION | $5,771,893 | D34 | published | science: research education | Bogart, GA | 2024 |
| LIONS TIGERS & BEARS | $5,435,303 | D34 | published | abuse: animal | Alpine, CA | 2024 |
| PROJECT CHIMPS | $5,388,549 | D34 | published | | Morgantown, GA | 2024 |
| SAVE THE CHIMPS INC | $5,265,228 | D34 | published | abuse: animal | Fort Pierce, FL | 2024 |
| TIGER HAVEN | $4,988,458 | D34 | published | abuse: animal | Kingston, TN | 2025 |
| OCEANIC SOCIETY EXPEDITIONS | $4,895,370 | D34 | inferred 0.50 | | Ross, CA | 2024 |
| FRIENDS OF THE SEA LION INC | $4,654,449 | D34 | published | science: research education | Laguna Beach, CA | 2024 |
| MIANUS RIVER GORGE INC | $4,518,012 | D34 | published | science: research education | Bedford, NY | 2025 |
| WILDLIFE CENTER OF VIRGINIA | $4,434,174 | D34 | published | domestic: animals | Waynesboro, VA | 2024 |
| GRIZZLY DISCOVERY CENTER A NOT- FOR-PROFIT CORPORATION | $4,360,552 | D34 | published | | W Yellowstone, MT | 2025 |
| WILDLIFE S O S | $4,259,217 | D34 | published | abuse: animal | Salt Lake City, UT | 2025 |
| THE BUSCH WILDLIFE SANCTUARY INC | $4,254,390 | D34 | published | | Jupiter, FL | 2024 |
| SPRING FARM CENTER ALTERNATIVE RESEARCH EDUCATION SANCTUARY | $3,585,739 | D34 | published | domestic: animals | Clinton, NY | 2025 |
| RUNNYMEDE SANCTUARY UA OF DIANA S WISTER 08052013 TR | $3,558,279 | D34 | published | | Coatesville, PA | 2024 |
| SANTA BARBARA WILDLIFE CARE NETWORK | $2,789,040 | D34 | published | | Goleta, CA | 2024 |
| VENTANA WILDLIFE SOCIETY | $2,656,010 | D34 | published | | Monterey, CA | 2024 |
| MINNESOTA VALLEY NATIONAL WILDLIFE REFUGE TRUST INC | $2,586,431 | D34 | published | | Bloomington, MN | 2025 |
| INSTITUTE OF RANGE & THE AMERICAN MUSTANG | $2,533,497 | D34 | published | | Hot Springs, SD | 2025 |
| SONOMA COUNTY WILDLIFE RESCUE | $2,514,571 | D34 | published | | Sonoma, CA | 2024 |
| WOODFORD CEDAR RUN WILDLIFE REFUGE INC | $2,391,744 | D34 | published | science: research education | Medford, NJ | 2025 |
| COLORADO WOLF AND WILDLIFE CENTER | $2,351,170 | D34 | published | | Divide, CO | 2024 |
| RIDGES SANCTUARY INC | $2,279,054 | D34 | published | | Baileys Harbor, WI | 2025 |
| CENTER FOR WILDLIFE INC | $2,273,689 | D34 | published | | Cape Neddick, ME | 2024 |
| CAROLINA TIGER RESCUE | $2,040,211 | D34 | published | | Pittsboro, NC | 2024 |
| AUSTIN WILDLIFE RESCUE | $1,923,082 | D34 | published | | Austin, TX | 2024 |
| GREATER OGDEN COMMUNITY NATURE CTR | $1,910,629 | D34 | published | science: research education | Ogden, UT | 2024 |
| CHIMPANZEE SANCTUARY NORTHWEST | $1,862,471 | D34 | published | abuse: animal | Cle Elum, WA | 2024 |
| AMERICAN YORKSHIRE CLUB INC | $1,861,820 | D34 | inferred 0.55 | | W Lafayette, IN | 2025 |
| OHIO WILDLIFE CENTER | $1,854,122 | D34 | published | domestic: animals | Powell, OH | 2025 |
| LION HABITAT RANCH INC | $1,834,127 | D34 | published | | Henderson, NV | 2025 |
| KAREN BEASLEY SEA TURTLE RESCUE AND REHABILITATION CENTER | $1,812,979 | D34 | published | | Surf City, NC | 2024 |
| GREENWOOD WILDLIFE REHABILITATION CENTER | $1,667,703 | D34 | published | | Boulder, CO | 2024 |
| TUCSON WILDLIFE CENTER | $1,633,300 | D34 | published | domestic: animals | Tucson, AZ | 2024 |
| THINK WILD | $1,584,407 | D34 | published | | Bend, OR | 2025 |
| TURKEY CREEK CONSERVATION AND RECREATION PRESERVE | $1,557,000 | D34 | published | | Bryan, TX | 2024 |
| WILDLIFE FOUNDATION OF VIRGINIA INC | $1,535,612 | D34 | published | | Norge, VA | 2024 |
| WILDLIFE IMAGES REHABILITATION AND EDUCATION CENTER | $1,509,069 | D34 | published | | Grants Pass, OR | 2024 |
| WILDCARE FOUNDATION | $1,508,131 | D34 | published | abuse: animal | Noble, OK | 2024 |
| EASTGATE EQUINE SANCTUARY INC | $1,382,044 | D34 | published | | Westport, CT | 2025 |
| AREA 51 SANCTUARY INC | $1,336,100 | D34 | published | | Schaumburg, IL | 2025 |
| FLAT ROCK BROOK NATURE ASSOCIATION | $1,308,673 | D34 | published | science: research education | Englewood, NJ | 2024 |
| PROFESSIONAL ANIMAL RETIREMENT CENTER | $1,307,470 | D34 | published | | Albion, IN | 2024 |
| FLORIDA WILDLIFE HOSPITAL AND SANCTUARY INC | $1,290,952 | D34 | published | | Melbourne, FL | 2025 |
| MISSOULA BUTTERFLY HOUSE AND INSECTARIUM | $1,281,878 | D34 | published | girls boys: girl scouts | Missoula, MT | 2024 |
| APPALACHIAN BLACK BEAR REHABILITATION AND RELEASE CENTER | $1,229,424 | D34 | published | | Townsend, TN | 2025 |
| ARTHURS ACRES ANIMAL SANCTUARY CORPORATION | $1,129,305 | D34 | published | abuse: animal | Parksville, NY | 2024 |
| AMERICAN FOUNDATION FOR WILDLIFE | $1,100,885 | D34 | published | | Bismarck, ND | 2024 |
| SOUL OF THE WOLF WILDLIFE SANCTUARY AND EDUCATIONAL FOUNDATION INC | $1,098,974 | D34 | published | abuse: animal | St Augustine, FL | 2025 |
| THE HUMANE SOCIETY WILDLIFE LAND TR | $1,094,491 | D34 | published | | Washington, DC | 2025 |
| WETLANDS AND WILDLIFE CARE CENTER | $1,039,845 | D34 | published | | Huntingtn Bch, CA | 2024 |
| POCONO WILDLIFE REHABILITATION CENTER | $1,020,883 | D34 | published | | Stroudsburg, PA | 2024 |
| AUSTIN FARM SANCTUARY | $1,016,114 | D34 | published | | Paige, TX | 2025 |
| PEACE RIVER WILDLIFE CENTER INC | $1,012,760 | D34 | published | | Punta Gorda, FL | 2024 |
| WARE FAMILY FOUNDATION INC | $997,791 | D34 | published | | Ponte Vedra, FL | 2025 |
| SHARON CHARITABLE TR | $970,828 | D34 | published | | Savannah, TN | 2024 |
| PEACE RIVER REFUGE & RANCH INC | $963,244 | D34 | published | abuse: animal | Silver Spgs, FL | 2024 |
| LITTLE SANDY HUNTING & FISHING CLUB | $961,488 | D34 | inferred 0.80 | | Hawkins, TX | 2024 |
| LIFE UNLIMITED OF VIRGINIA INC | $958,789 | D34 | published | abuse: animal | Orange, VA | 2024 |
| SHARKAROSA RANCH | $930,561 | D34 | published | | Pilot Point, TX | 2025 |
| TIGER PRESERVATION CENTER NEVADA | $915,316 | D34 | published | abuse: animal | Cave Junction, OR | 2025 |
| WILD AT HEART INC | $890,575 | D34 | published | | Cave Creek, AZ | 2024 |
| PERCHERON HORSE ASSN OF AMERICA | $863,738 | D34 | inferred 0.50 | | Fredericktown, OH | 2024 |
| RIVERHEAD FOUNDATION FOR MARINE RESEARCH AND PRESERVATION | $860,203 | D34 | published | | Riverhead, NY | 2024 |
| MARYLAND SHEEP BREEDERS ASSOC INC | $857,354 | D34 | inferred 0.50 | | Finksburg, MD | 2024 |
| ROGERS WILDLIFE REHABILITATION INC | $851,111 | D34 | published | | Hutchins, TX | 2025 |
| WALDENS PUDDLE INC | $848,344 | D34 | published | girls boys: boy scouts | Joelton, TN | 2025 |
| CATTY SHACK RANCH WILDLIFE SANCTUARY INC | $843,770 | D34 | published | | Jacksonville, FL | 2024 |
| TORDIK WILDLIFE FOUNDATION INC | $838,289 | D34 | inferred 0.75 | | Carter, MT | 2025 |
| FRANK STEELE FOUNDATION INC | $834,331 | D34 | published | domestic: animals | Geary, OK | 2025 |
| WOLF HOLLOW WILDLIFE REHABILITATION CENTRE | $825,977 | D34 | published | | Friday Harbor, WA | 2024 |
| NEWHOUSE WILDLIFE RESCUE CORPORATION | $799,806 | D34 | published | | Chelmsford, MA | 2025 |
| RED CARIBENA DE VARAMIENTOS -CARIBBEAN STRANDING NETWORK INC- | $776,067 | D34 | published | | San Juan, PR | 2025 |
| MCCARTHYS WILDLIFE SANCTUARY INC | $768,209 | D34 | published | | West Palm Bch, FL | 2025 |
| CHINTIMINI WILDLIFE CENTER | $760,807 | D34 | published | | Corvallis, OR | 2024 |
| JACKSON HOLE WILDLIFE FOUNDATION | $726,156 | D34 | published | science: research education | Jackson, WY | 2025 |
| HAWK CREEK WILDLIFE CENTER | $719,385 | D34 | published | | East Aurora, NY | 2025 |
| WILD SPIRIT WOLF SANCTUARY | $709,923 | D34 | published | | Ramah, NM | 2023 |
| HOFFMAN CENTER | $700,000 | D34 | published | | Muttontown, NY | 2024 |
| NORTH SHORE WILDLIFE SANCTUARY INC | $695,522 | D34 | published | | Bayville, NY | 2024 |
| CENTRAL FLORIDA ANIMAL RESERVE INC | $691,399 | D34 | published | | Saint Cloud, FL | 2024 |
| NATURES NURSERY CENTER FOR WILDLIFE REHABILITATION AND CONSERVATION | $688,768 | D34 | published | | Waterville, OH | 2024 |
| ORANGUTAN OUTREACH INC | $677,251 | D34 | published | | Earlton, NY | 2025 |
| APEX PROTECTION PROJECT | $652,214 | D34 | published | shelter: animal | Palmdale, CA | 2024 |
| CONSERVATORS CENTER INC | $647,779 | D34 | published | | Mebane, NC | 2025 |
| WILDLIFE SANCTUARY OF NORTHWEST FLORIDA INC | $635,727 | D34 | published | | Pensacola, FL | 2025 |
| WILD WINGS INC | $635,725 | D34 | published | shelter: animal | Honeoye Falls, NY | 2025 |
| ALABAMA WILDLIFE CENTER | $604,582 | D34 | published | | Pelham, AL | 2024 |
| WILDWOODS | $601,020 | D34 | published | | Duluth, MN | 2025 |
| PRIMATE RESCUE CENTER INC | $594,326 | D34 | published | shelter: animal | Nicholasville, KY | 2024 |
| ROCKFISH WILDLIFE SANCTUARY INC | $567,609 | D34 | published | | Shipman, VA | 2025 |
| CHAGRIN VALLEY PHA HORSE SHOWS INC | $559,432 | D34 | published | | Chagrin Falls, OH | 2025 |