| THE MITRE CORPORATION | $2,475,879,129 | U40 | published | science: research education | Bedford, MA | 2024 |
| CHARLES STARK DRAPER LABORATORY INC | $949,348,102 | U40 | published | launch: space | Cambridge, MA | 2024 |
| BLUEFORGE ALLIANCE | $731,167,389 | U40 | published | science: research education | Bryan, TX | 2024 |
| PREPASS SAFETY ALLIANCE | $196,625,833 | U40 | published | | Phoenix, AZ | 2024 |
| DEFENSEWERX INC | $195,761,877 | U40 | published | science: research education | Niceville, FL | 2024 |
| THE HAWAII PACIFIC FOUNDATION INC | $173,736,286 | U40 | published | abuse: substance | Honolulu, HI | 2023 |
| NY CREATES | $144,806,088 | U40 | published | science: research education | Albany, NY | 2024 |
| ANALYTIC SERVICES INC | $142,473,079 | U40 | published | science: research education | Arlington, VA | 2024 |
| NATIONAL ADVANCED MOBILITY CONSORTIUM | $135,705,794 | U40 | published | | Ann Arbor, MI | 2024 |
| AMERICAN SOCIETY FOR TESTING AND MATERIALS | $134,694,228 | U40 | published | launch: space | Conshohocken, PA | 2024 |
| NATIONAL CENTER FOR DEFENSE MANUFACTURING AND MACHINING | $119,433,940 | U40 | published | science: research education | Johnstown, PA | 2024 |
| CONCURRENT TECHNOLOGIES CORPORATION | $101,274,766 | U40 | published | space: outer | Johnstown, PA | 2024 |
| FOR INSPIRATION AND RECOGNITION OF SCIENCE AND TECHNOLOGY FIRST | $84,935,359 | U40 | published | launch: space | Manchester, NH | 2024 |
| MCNC | $74,135,271 | U40 | published | science: research education | R Triangle Park, NC | 2024 |
| AMERICAN LIGHTWEIGHT MATERIALS MANUFACTURING INNOVATION INSTITUTE | $42,468,343 | U40 | published | science: research education | Detroit, MI | 2024 |
| MOZILLA FOUNDATION | $37,165,545 | U40 | published | men: issues | San Francisco, CA | 2024 |
| ANITA BORG INSTITUTE FOR WOMEN AND TECHNOLOGY | $27,514,949 | U40 | published | science: research education | Sacramento, CA | 2024 |
| FAR AI INC | $24,293,765 | U40 | published | | San Diego, CA | 2024 |
| COMMONWEALTH CENTER FOR ADVANCED MANUFACTURING | $23,287,216 | U40 | published | space: outer | Disputanta, VA | 2024 |
| NATIONAL SOCIETY OF BLACK ENGINEERS | $22,741,085 | U40 | published | science: research education | Alexandria, VA | 2024 |
| SOUTH CAROLINA RESEARCH AUTHORITY | $16,234,461 | U40 | published | science: research education | Columbia, SC | 2022 |
| TECHNICAL ASSOCIATION OF THE PULP & PAPER INDUSTRY INC | $15,876,580 | U40 | published | | Peachtree Corners, GA | 2024 |
| STEPHENSON STELLAR CORPORATION | $15,638,567 | U40 | published | science: research education | Shreveport, LA | 2024 |
| TERC INC | $14,400,977 | U40 | published | science: research education | Cambridge, MA | 2024 |
| CATALYST CONNECTION | $13,475,224 | U40 | published | | Pittsburgh, PA | 2024 |
| CLARK RICHARDSON AND BISKUP CONSULTING ENGINEERS INC HEALTH PL | $13,397,579 | U40 | published | | Kansas City, MO | 2024 |
| CENTER FOR ENVIRONMENTAL HEALTH MONITORING AND MANAGEMENT | $13,092,596 | U40 | published | | Carlsbad, NM | 2024 |
| BIOONE | $12,823,538 | U40 | published | science: research education | Washington, DC | 2025 |
| NATIONAL LAMBDARAIL INC (NLR INC) | $11,703,979 | U40 | inferred 0.60 | | Culver City, CA | 2013 |
| ANALYSIS AND RESILIENCE CENTER FOR SYSTEMIC RISK INC | $11,544,274 | U40 | published | | Arlington, VA | 2025 |
| INTERNATIONAL FIRST COMMITTEE ASSOCIATION | $11,185,122 | U40 | published | science: research education | Manchester, NH | 2024 |
| NORTHWEST OHIO INNOVATION CONSORTIUM INC | $10,151,834 | U40 | published | | Perrysburg, OH | 2024 |
| THE FORESIGHT INSTITUTE | $9,356,634 | U40 | published | space: outer | San Francisco, CA | 2024 |
| APPLIED SCIENCE AND TECHNOLOGY RESEARCH ORGANIZATION OF AMERICS | $8,783,704 | U40 | published | space: outer | Moline, IL | 2024 |
| WORLD WIDE WEB CONSORTIUM INC | $8,709,387 | U40 | published | | Wakefield, MA | 2024 |
| INSTITUTE OF TRANSPORTATION ENGINEERS INC | $8,189,181 | U40 | published | science: research education | Washington, DC | 2024 |
| OUGHT INC | $8,185,576 | U40 | published | science: research education | Covina, CA | 2024 |
| HOUSTON ADVANCED RESEARCH CENTER | $7,838,125 | U40 | published | | Spring, TX | 2024 |
| BRINK TECHNOLOGY INC | $6,733,129 | U40 | published | | Austin, TX | 2024 |
| MATS RESEARCH INC | $6,308,393 | U40 | published | | Berkeley, CA | 2024 |
| ENGINEERING CONFERENCES INTERNATIONAL INC | $5,934,887 | U40 | published | science: research education | New York, NY | 2024 |
| AQUAYA INSTITUTE | $5,647,416 | U40 | published | science: research education | Fairfax, CA | 2025 |
| ALIGNMENT RESEARCH CENTER | $5,219,369 | U40 | published | | Covina, CA | 2024 |
| THE ASME FOUNDATION INC | $5,191,568 | U40 | published | space: outer | New York, NY | 2024 |
| MILITARY HEALTH RESEARCH FOUNDATION INC | $5,110,658 | U40 | published | science: research education | Laurel, MD | 2024 |
| BASIS RESEARCH INSTITUTE | $4,745,774 | U40 | published | | New York, NY | 2024 |
| BUILD CHANGE | $4,595,428 | U40 | published | shelter: disaster | Denver, CO | 2024 |
| APPLIED TECHNOLOGY COUNCIL | $4,434,238 | U40 | published | | Redwood City, CA | 2024 |
| TECHNOLOGY MATTERS | $4,193,112 | U40 | published | | Palo Alto, CA | 2024 |
| THE SOCIETY OF HISPANIC PROFESSIONAL | $4,024,126 | U40 | inferred 0.60 | science: research education | City Of Industry, CA | 2013 |
| LOUISIANA TECH APPLIED RESEARCH CORPORATION | $3,684,037 | U40 | published | | Bossier City, LA | 2024 |
| WIRELESS RESEARCH CENTER OF NORTH CAROLINA | $3,663,865 | U40 | published | | Wake Forest, NC | 2024 |
| CORTICO CORPORATION | $3,634,804 | U40 | published | | Boston, MA | 2024 |
| BATTERY INNOVATION CENTER INSTITUTE INC | $3,271,408 | U40 | published | | Newberry, IN | 2024 |
| PALISADE RESEARCH INC | $3,232,257 | U40 | published | | Dover, DE | 2024 |
| AMERICAN CONFERENCE OF GOVERNMENTAL INDUSTRIAL HYGIENISTS INC | $2,799,768 | U40 | published | science: research education | Sharonville, OH | 2024 |
| LASER INSTITUTE OF AMERICA | $2,642,681 | U40 | published | | Orlando, FL | 2024 |
| RESPONSIBLE INNOVATION LABS INC | $2,490,600 | U40 | published | | Dover, DE | 2024 |
| GLOBALPLATFORM INC A DELAWARE CORPORATION | $2,420,756 | U40 | published | | Redwood City, CA | 2024 |
| OPENJS FOUNDATION | $2,412,467 | U40 | inferred 0.50 | | Wilmington, DE | 2024 |
| NUCLEAR MATTERS | $2,309,945 | U40 | published | | Washington, DC | 2024 |
| 4YOUANDME | $2,307,053 | U40 | published | | Great Neck, NY | 2024 |
| BGH FOUNDATION | $2,237,108 | U40 | published | | Fredericksbrg, VA | 2024 |
| WESTERN NEW YORK TECHNOLOGY DEVELOPMENT CENTER INC | $2,149,736 | U40 | published | | Buffalo, NY | 2024 |
| ADDITIVE MANUFACTURING USERS GROUP INC | $2,085,702 | U40 | published | | Belton, TX | 2024 |
| ASSOCIATION OF TECHNOLOGY LEADERS IN INDEPENDENT SCHOOLS | $2,047,264 | U40 | published | | Washington, DC | 2025 |
| SYMBOL SYNDICATE | $1,996,291 | U40 | published | | Kirkland, WA | 2022 |
| AMERICAN INSTITUTE OF MINING METALLURGICAL AND PETROLEUM ENGINE | $1,988,585 | U40 | published | science: research education | San Ramon, CA | 2024 |
| ST LOUIS REGIONAL ADVANCED MANUFACTURING INNOVATION CENTER | $1,924,781 | U40 | published | | Clayton, MO | 2024 |
| SAKAI FOUNDATION | $1,825,909 | U40 | inferred 0.60 | | Ann Arbor, MI | 2012 |
| STEPHENSON TECHNOLOGIES CORPORATION | $1,736,246 | U40 | published | science: research education | Baton Rouge, LA | 2024 |
| UNIVERSAL CHIPLET INTERCONNECT EXPRESS INC | $1,622,931 | U40 | published | | Beaverton, OR | 2025 |
| OPEN ENVIRONMENTAL DATA INC | $1,582,088 | U40 | published | | Dover, DE | 2025 |
| WEPAN | $1,542,750 | U40 | published | space: for women | Washington, DC | 2024 |
| AMERICAN AUTOMATIC CONTROL COUNCIL | $1,542,253 | U40 | published | science: research education | Seattle, WA | 2024 |
| HEARTLAND SCIENCE AND TECHNOLOGY GROUP | $1,440,622 | U40 | published | science: research education | Chillicothe, IL | 2025 |
| SPACETEC PARTNERS INC | $1,439,425 | U40 | published | space: outer | Titusville, FL | 2024 |
| AMERICAN COUNCIL OF ENGINEERING COMPAINIES OF TENNESSEE INC | $1,411,914 | U40 | published | | Nashville, TN | 2024 |
| ACI CENTER OF EXCELLENCE FOR NONMETALLICS IN BUILDING MATERIALS | $1,343,553 | U40 | published | | Farmington Hills, MI | 2025 |
| ASSOCIATION FOR INFORMATION SCIENCE & TECHNOLOGY | $1,317,904 | U40 | published | science: research education | Roseville, MN | 2024 |
| INSTITUTE OF DIGITAL ENGINEERING USA | $1,292,945 | U40 | published | | Mooresville, NC | 2025 |
| PETROLEUM TECHNOLOGY TRANSFER COUNCIL | $1,215,684 | U40 | inferred 0.50 | | Oak Hill, VA | 2009 |
| VERMONT CENTER FOR GEOGRAPHIC INFORMATION INC | $1,184,449 | U40 | inferred 0.50 | | Waterbury, VT | 2013 |
| OREGON UAS ACCELERATOR | $1,088,654 | U40 | published | | Pendleton, OR | 2025 |
| DAKOTA STATE UNIVERSITY APPLIED RESEARCH CORPORATION | $1,070,000 | U40 | published | | Madison, SD | 2024 |
| BROKAW FAMILY FOUNDATION | $1,065,861 | U40 | published | | Merrimack, NH | 2024 |
| ALEK KAVCIC FOUNDATION | $1,054,367 | U40 | published | science: research education | Austin, TX | 2024 |
| SOCIAL ALPHA INNOVATION FOUNDATION | $1,029,455 | U40 | published | | Saratoga, CA | 2024 |
| INSTITUTE FOR TECHNOLOGY DEVELOPMENT INC | $1,013,672 | U40 | published | space: outer | Ridgeland, MS | 2024 |
| INTERNATIONAL SOCIETY OF OFFSHORE AND POLAR ENGINEERS | $938,192 | U40 | published | | Mountain View, CA | 2024 |
| GEORGIAFIRST ROBOTICS INC | $923,212 | U40 | published | science: research education | Cumming, GA | 2024 |
| SEMIO COMMUNITY | $901,676 | U40 | published | | Los Angeles, CA | 2025 |
| LA-MAS INC | $899,335 | U40 | published | | Los Angeles, CA | 2024 |
| UNLV RESEARCH FOUNDATION | $832,706 | U40 | published | | Las Vegas, NV | 2024 |
| JOHNSON STEM ACTIVITY CENTER INC | $810,455 | U40 | published | | Atlanta, GA | 2024 |
| FRICTION MATERIALS STANDARDS | $738,661 | U40 | published | | Auburn Hills, MI | 2024 |
| REINHARDT | $730,000 | U40 | published | science: research education | Huntsville, AL | 2025 |
| KOREA US QUANTUM TECHNOLOGY COOPERATION CENTER | $729,934 | U40 | published | | Vienna, VA | 2024 |
| HOTROCK ENERGY RESEARCH ORGANIZATION | $712,089 | U40 | published | | Seattle, WA | 2025 |
| OPENAI INC | $691,271 | U40 | published | | San Francisco, CA | 2024 |