| SMITHSONIAN INSTITUTE | $1,911,142,906 | A50 | published | space: outer | Washington, DC | 2024 |
| NATIONAL FOUNDATION FOR THE CTRS FOR DISEASE CONTR & PREVENTION INC | $165,571,412 | A50 | published | violence: gun | Atlanta, GA | 2024 |
| LUCAS MUSEUM OF NARRATIVE ART | $158,337,473 | A50 | published | | Los Angeles, CA | 2024 |
| CARNEGIE INSTITUTE | $115,993,787 | A50 | published | space: outer | Pittsburgh, PA | 2024 |
| WISCONSIN MUSEUM OF NATURE AND CULTURE INC | $103,029,479 | A50 | published | | Milwaukee, WI | 2024 |
| HISTORIC HAYMARKET MILWAUKEE I INC | $75,670,824 | A50 | published | launch: launch | Milwaukee, WI | 2025 |
| ROYAL ONTARIO MUSEUM | $74,502,137 | A50 | published | | Canada | 2024 |
| CROSSWATER CANYON INC | $74,260,393 | A50 | published | | Petersburg, KY | 2024 |
| COUNTRY MUSIC FOUNDATION INC | $73,334,385 | A50 | published | | Nashville, TN | 2024 |
| KOHLER FOUNDATION INC | $70,543,708 | A50 | inferred 0.60 | girls boys: girl scouts | Kohler, WI | 2025 |
| THE EXPLORATORIUM | $63,991,919 | A50 | published | girls boys: boys girls clubs | San Francisco, CA | 2024 |
| THE STUDIO MUSEUM IN HARLEM | $61,749,837 | A50 | published | women: issues | New York, NY | 2024 |
| ROCK AND ROLL HALL OF FAME AND MUSEUM INC | $57,541,431 | A50 | published | girls boys: girl scouts | Cleveland, OH | 2025 |
| THE CICI AND HYATT BROWN MUSEUM OF ART SCIENCE & HISTORY INC | $51,560,135 | A50 | published | space: outer | Daytona Beach, FL | 2025 |
| ANGELA DULDULAO MCPHERSON FOUNDATION | $49,690,241 | A50 | published | | Oxnard, CA | 2024 |
| MUSEUM OF CHINESE IN THE AMERICA | $43,284,661 | A50 | published | | New York, NY | 2024 |
| MEDAL OF HONOR MUSEUM FOUNDATION | $43,221,323 | A50 | published | | Arlington, TX | 2024 |
| BRANDYWINE CONSERVANCY & MUSEUM OF ART | $42,707,848 | A50 | published | | Chadds Ford, PA | 2024 |
| MUSICAL INSTRUMENT MUSEUM | $41,100,797 | A50 | published | | Phoenix, AZ | 2024 |
| USS MIDWAY MUSEUM | $39,443,527 | A50 | published | | San Diego, CA | 2024 |
| INTREPID MUSEUM FOUNDATION INC | $39,353,836 | A50 | published | launch: space | New York, NY | 2024 |
| MARY AND JACKSON BURKE FOUNDATION | $39,212,428 | A50 | inferred 0.60 | | St Paul, MN | 2015 |
| OUR RESCUE | $35,421,220 | A50 | published | abuse: sexual | Sandy, UT | 2025 |
| NATIONAL FOOTBALL MUSEUM INC | $35,346,792 | A50 | published | | Canton, OH | 2024 |
| CINCINNATI MUSEUM CENTER | $34,467,370 | A50 | published | science: research education | Cincinnati, OH | 2024 |
| JEWISH MUSEUM | $33,802,678 | A50 | published | | New York, NY | 2024 |
| INTERNATIONAL SPY MUSEUM | $31,785,524 | A50 | published | science: research education | Washington, DC | 2024 |
| PETERSEN AUTOMOTIVE MUSEUM FOUNDATION | $31,661,696 | A50 | published | | Los Angeles, CA | 2025 |
| COMMEMORATIVE AIR FORCE | $30,020,664 | A50 | published | | Dallas, TX | 2024 |
| MONA BISMARCK AMERICAN CENTER FOR ART | $27,776,617 | A50 | inferred 0.50 | | New York, NY | 2023 |
| PEABODY ESSEX MUSEUM INC | $26,403,862 | A50 | published | science: research education | Salem, MA | 2024 |
| JOHNNY MORRIS WONDERS OF WILDLIFE FOUNDATION | $26,281,365 | A50 | published | | Springfield, MO | 2025 |
| NATIONAL MUSEUM OF MATHEMATICS | $25,872,371 | A50 | published | science: research education | New York, NY | 2024 |
| MYSTIC SEAPORT MUSEUM INC | $25,343,759 | A50 | published | space: outer | Mystic, CT | 2024 |
| CHARLES & EMMA FRYE FREE PUBLIC ART MUSEUM INC TESTAMENTARY TRUST | $23,949,445 | A50 | inferred 0.85 | | Seattle, WA | 2024 |
| NEWARK MUSEUM ASSOCIATION | $23,750,238 | A50 | published | launch: space | Newark, NJ | 2024 |
| HEARD MUSEUM | $23,249,082 | A50 | published | | Phoenix, AZ | 2024 |
| INDIANAPOLIS MOTOR SPEEDWAY FOUNDATION INC | $22,026,626 | A50 | inferred 0.85 | | Indianapolis, IN | 2025 |
| ADLER PLANETARIUM | $21,973,192 | A50 | published | space: outer | Chicago, IL | 2024 |
| DISCOVERY SCIENCE CENTER OF ORANGE COUNTY | $21,458,282 | A50 | published | science: research education | Santa Ana, CA | 2024 |
| EXPERIENCE LEARNING COMMUNITY | $21,040,617 | A50 | published | | Seattle, WA | 2024 |
| THE MARITIME AQUARIUM AT NORWALK INC | $20,483,968 | A50 | published | science: research education | South Norwalk, CT | 2024 |
| GRAND RAPIDS PUBLIC MUSEUM | $19,808,927 | A50 | published | space: outer | Grand Rapids, MI | 2024 |
| NATIONAL CORVETTE MUSEUM FOUNDATION INC | $19,478,552 | A50 | published | | Bowling Green, KY | 2024 |
| BARBER VINTAGE MOTORSPORTS MUSEUM | $19,469,927 | A50 | published | science: research education | Birmingham, AL | 2025 |
| SHELBURNE MUSEUM INCORPORATED | $19,058,612 | A50 | published | | Shelburne, VT | 2024 |
| OAKLAND MUSEUM OF CALIFORNIA | $17,373,397 | A50 | published | launch: launch | Oakland, CA | 2024 |
| THE PALEY CENTER FOR MEDIA | $17,189,064 | A50 | published | | Newyork, NY | 2024 |
| HOLOCAUST MUSEUM LA | $16,029,822 | A50 | published | | Los Angeles, CA | 2024 |
| WITTE MUSEUM | $15,651,743 | A50 | published | science: research education | San Antonio, TX | 2024 |
| EXPLORATION PLACE INC | $14,988,402 | A50 | published | science: research education | Wichita, KS | 2024 |
| GRAMMY MUSEUM FOUNDATION INC | $14,969,268 | A50 | published | science: research education | Los Angeles, CA | 2024 |
| MARINERS MUSEUM | $14,341,457 | A50 | published | science: research education | Newport News, VA | 2024 |
| GEORGE EASTMAN MUSEUM | $14,339,669 | A50 | published | science: research education | Rochester, NY | 2024 |
| PALM BEACH MARITIME MUSEUM INC | $14,134,905 | A50 | published | science: research education | Lantana, FL | 2024 |
| BELLWETHER FOUNDATION INC | $13,985,350 | A50 | inferred 0.55 | girls boys: girls | Saint Louis, MO | 2025 |
| EITELJORG MUSEUM OF AMERICAN INDIANS AND WESTERN ART INC | $13,581,775 | A50 | published | girls boys: boy scouts | Indianapolis, IN | 2024 |
| NAVAL AVIATION MUSEUM FOUNDATION INC | $13,361,566 | A50 | published | launch: aviation | Pensacola, FL | 2025 |
| GEORGIA MUSEUMS INC | $13,338,398 | A50 | published | space: outer | Cartersville, GA | 2024 |
| MILWAUKEE PUBLIC MUSEUM INC | $13,076,645 | A50 | published | science: research education | Milwaukee, WI | 2024 |
| HOLOCAUST MUSEUM HOUSTON | $12,703,476 | A50 | published | girls boys: girl scouts | Houston, TX | 2024 |
| STAFFORD AIR & SPACE MUSEUM FOUNDATION | $12,374,396 | A50 | published | space: outer | Weatherford, OK | 2024 |
| MUSEUM OF AFRICAN AMERICAN HISTORY | $12,370,555 | A50 | published | | Detroit, MI | 2024 |
| CHESAPEAKE BAY MARITIME MUSEUM INC | $12,307,465 | A50 | published | | St Michaels, MD | 2025 |
| JAPANESE AMERICAN NATIONAL MUSEUM | $12,194,816 | A50 | published | girls boys: girl scouts | Los Angeles, CA | 2024 |
| SCIENCE MUSEUM OKLAHOMA INC | $12,130,425 | A50 | published | space: outer | Oklahoma City, OK | 2024 |
| AMERICAN MUSEUM OF THE MOVING IMAGE | $12,101,609 | A50 | published | space: outer | Astoria, NY | 2024 |
| GRAND RAPIDS PUBLIC MUSEUM FOUNDATION | $12,022,942 | A50 | published | | Grand Rapids, MI | 2024 |
| NAISMITH MEMORIAL BASKETBALL HALL OF FAME INC 1150 WEST COLUMBUS AVE | $11,864,912 | A50 | published | | Springfield, MA | 2024 |
| PEARL HARBOR AVIATION MUSEUM | $11,844,712 | A50 | published | | Honolulu, HI | 2024 |
| NATIONAL BUILDING MUSEUM | $11,722,698 | A50 | published | violence: gun | Washington, DC | 2024 |
| NATIONAL CENTER FOR CIVIL AND HUMANRIGHTS INC | $11,504,595 | A50 | published | launch: launch | Atlanta, GA | 2024 |
| THE NATIONAL COWGIRL MUSEUM AND HALL OF FAME INC | $11,485,731 | A50 | published | women: issues | Fort Worth, TX | 2024 |
| DISCOVERY PARK OF AMERICA INC | $11,393,188 | A50 | published | science: research education | Union City, TN | 2024 |
| INTERNATIONAL AFRICAN AMERICAN MUSEUM | $11,151,191 | A50 | published | | Charleston, SC | 2024 |
| HISTORIC ARNOLDS PARK INC | $11,094,253 | A50 | published | | Arnolds Park, IA | 2024 |
| SUN N FUN FLY IN INC | $11,079,293 | A50 | published | space: outer | Lakeland, FL | 2024 |
| FORT WORTH MUSEUM OF SCIENCE AND HISTORY | $11,013,898 | A50 | published | space: outer | Fort Worth, TX | 2024 |
| CONRU ART FOUNDATION TR | $10,972,686 | A50 | published | | Minneapolis, MN | 2024 |
| ATLANTA HALL MANAGEMENT INC | $10,806,827 | A50 | published | | Atlanta, GA | 2024 |
| COLUMBIA RIVER MARITIME MUSEUM INC | $10,765,579 | A50 | published | | Astoria, OR | 2024 |
| WJCT INC | $10,756,730 | A50 | inferred 0.60 | women: issues | Jacksonville, FL | 2024 |
| MINGEI INTERNATIONAL INC | $10,512,072 | A50 | published | | San Diego, CA | 2024 |
| WEITZMAN NATIONAL MUSEUM OF AMERICAN JEWISH HISTORY | $10,343,108 | A50 | published | | Philadelphia, PA | 2024 |
| GILCREASE MUSEUM MANAGEMENT TRUST | $9,890,593 | A50 | published | | Tulsa, OK | 2024 |
| MARIETTA MUSEUM OF ART & WHIMSY INC | $9,850,836 | A50 | published | | Sarasota, FL | 2025 |
| PACIFIC FLEET SUBMARINE MEMORIAL ASSOCIATION INC | $9,802,795 | A50 | published | | Honolulu, HI | 2024 |
| NEGRO LEAGUES BASEBALL MUSEUM INC | $9,801,523 | A50 | published | | Kansas City, MO | 2024 |
| LOCKWOOD-MATHEWS MANSION MUSEUM OF NORWALK INC | $9,788,543 | A50 | published | | Norwalk, CT | 2024 |
| PALM SPRINGS ART MUSEUM | $9,732,898 | A50 | published | | Palm Springs, CA | 2023 |
| ROCK & ROLL HALL OF FAME FOUNDATION INC | $9,574,505 | A50 | published | | New York, NY | 2024 |
| STONE BARNS RESTORATION CORP | $9,517,627 | A50 | published | science: research education | Tarrytown, NY | 2024 |
| SAN DIEGO HISTORICAL SOCIETY | $9,316,303 | A50 | inferred 0.85 | women: issues | San Diego, CA | 2024 |
| EDWARD JOHN NOBLE FOUNDATION INC | $9,293,982 | A50 | inferred 0.40 | abuse: child | Greenwich, CT | 2024 |
| WENATCHEE VALLEY MUSEUM AND CULTURAL CENTER | $9,227,841 | A50 | published | space: outer | Wenatchee, WA | 2024 |
| NATIONAL MUSEUM OF WILDLIFE ART OF THE UNITED STATES | $9,193,589 | A50 | published | domestic: animals | Jackson, WY | 2024 |
| CHILDRENS MUSEUM OF PHOENIX | $9,101,352 | A50 | published | abuse: child | Phoenix, AZ | 2024 |
| NEON MUSEUM | $9,097,754 | A50 | published | | Las Vegas, NV | 2024 |
| TENNESSEE VALLEY RAILROAD MUSEUM INC | $8,937,912 | A50 | published | | Chattanooga, TN | 2024 |
| CHILDRENS MUSEUM OF NORTHWESTARKANSAS | $8,871,633 | A50 | published | science: research education | Bentonville, AR | 2024 |