| NATIONAL CENTER FOR MANUFACTURING SCIENCES | $568,204,237 | S40 | published | space: outer | Ann Arbor, MI | 2024 |
| BIOINDUSTRIAL MANUFACTURING AND DESIGN ECOSYSTEM | $96,121,380 | S40 | published | | Emeryville, CA | 2024 |
| CONFERENCE BOARD INC | $95,469,652 | S40 | published | | New York, NY | 2024 |
| SOUNDEXCHANGE INC | $95,229,752 | S40 | published | | Washington, DC | 2024 |
| UNITED STATES SOYBEAN EXPORT COUNCIL INC | $79,095,999 | S40 | published | | Chesterfield, MO | 2024 |
| GREEN BUSINESS CERTIFICATION INC | $77,471,030 | S40 | published | | Washington, DC | 2024 |
| APPLE INDUSTRIAL DEVELOPMENT CORP | $59,908,872 | S40 | inferred 0.50 | | New York, NY | 2014 |
| SECURITIES INDUSTRY AND FINANCIAL MARKETS ASSOCIATION INC | $56,703,129 | S40 | published | abuse: elder | New York, NY | 2024 |
| CIRCUIT EVENTS LOCAL ORGANIZING COMMITTEE | $54,145,048 | S40 | published | | Del Valle, TX | 2024 |
| MINNESOTA SUPER BOWL HOST COMMITTEE INC | $53,925,011 | S40 | inferred 0.55 | | Minneapolis, MN | 2018 |
| SAN DIEGO TOURISM MARKETING DISTRICT | $52,841,932 | S40 | published | | San Diego, CA | 2024 |
| VIRGINIA EQUINE ALLIANCE | $40,623,195 | S40 | published | abuse: animal | Warrenton, VA | 2025 |
| NEW ORLEANS PHYSICIAN SERVICES INC | $40,281,920 | S40 | published | | Marrero, LA | 2024 |
| BAY AREA HOST COMMITTEE | $38,680,341 | S40 | published | | Mountain View, CA | 2024 |
| LTC REINSURANCE PCC | $34,299,752 | S40 | published | | Washington, DC | 2025 |
| WISCONSIN MILK MARKETING BOARD INC | $32,730,107 | S40 | inferred 0.60 | science: research education | Madison, WI | 2024 |
| URAC | $31,698,569 | S40 | published | | Washington, DC | 2025 |
| MIDWAY AIRLINES TERMINAL CONSORTIUM | $30,606,988 | S40 | published | | Chicago, IL | 2024 |
| ORION INDUSTRIES | $30,468,783 | S40 | published | space: outer | Auburn, WA | 2024 |
| SIGNAL TECHNOLOGY FOUNDATION | $29,413,537 | S40 | published | | Mountain View, CA | 2024 |
| MIDWEST MINNESOTA COMMUNITY DEVELOPMENT CORPORATION | $25,316,666 | S40 | published | | Detroit Lakes, MN | 2024 |
| DALLAS TOURISM PUBLIC IMPROVEMENT DISTRICT CORPORATION | $25,159,301 | S40 | published | | Dallas, TX | 2024 |
| BETTER BUSINESS BUREAU GREAT WEST & PACIFIC INC | $22,566,911 | S40 | inferred 0.85 | | Meridian, ID | 2024 |
| DBIA SERVICES | $22,214,397 | S40 | published | | Seattle, WA | 2024 |
| SOUTHEASTERN NEW ENGLAND DEFENSE INDUSTRY ALLIANCE | $21,601,349 | S40 | published | science: research education | Middletown, RI | 2024 |
| OHIO COMMUNITY DEVELOPMENT FINANCE FUND | $20,728,998 | S40 | published | | Columbus, OH | 2024 |
| INTERNATIONAL ACCREDITATION SERVICE INC | $20,643,157 | S40 | published | | Cntry Clb Hls, IL | 2024 |
| AMERICAN PRODUCTIVITY & QUALITY CENTER INC | $20,610,235 | S40 | published | | Houston, TX | 2024 |
| OPEN COMPUTE PROJECT FOUNDATION | $18,947,048 | S40 | published | | Austin, TX | 2024 |
| 34TH STREET PARTNERSHIP INC | $16,974,657 | S40 | published | | New York, NY | 2024 |
| MEAT INSTITUTE | $16,814,686 | S40 | published | | Arlington, VA | 2024 |
| STRUCTURED FINANCE INDUSTRY GROUP INC | $15,423,194 | S40 | published | | Washington, DC | 2024 |
| INITIATIVE FOUNDATION | $15,215,240 | S40 | published | violence: sexual | Little Falls, MN | 2024 |
| ASCENDUS INC | $14,826,378 | S40 | published | | New York, NY | 2025 |
| SOUTHWEST INITIATIVE FOUNDATION | $14,711,866 | S40 | published | launch: aviation | Hutchinson, MN | 2024 |
| BRIDGEWAY CAPITAL INC | $14,120,469 | S40 | published | | Pittsburgh, PA | 2024 |
| GRAND CENTRAL PARTNERSHIP INC | $14,093,577 | S40 | published | | New York, NY | 2024 |
| ORANGE COUNTY REALTORS | $13,573,278 | S40 | inferred 0.85 | | Laguna Hills, CA | 2024 |
| CENTURY ASSOCIATION | $13,137,320 | S40 | inferred 0.85 | | New York, NY | 2024 |
| RUSSELL INNOVATION CENTER FOR ENTREPRENEURS INC | $12,084,148 | S40 | published | | Atlanta, GA | 2024 |
| TAX EXECUTIVES INSTITUTE INC | $11,773,163 | S40 | inferred 0.45 | | Washington, DC | 2024 |
| NONPROFITS UNITED | $11,574,060 | S40 | published | | Sacramento, CA | 2025 |
| VIRGINIA UTILITY PROTECTION SERVICE INC | $11,297,876 | S40 | published | | Roanoke, VA | 2025 |
| SAN ANTONIO TOURISM PUBLIC IMPROVEMENT DISTRICT CORPORATION | $11,108,577 | S40 | published | | San Antonio, TX | 2024 |
| ROCHESTER RIVERSIDE CONVENTION CTR | $10,971,379 | S40 | inferred 0.60 | | Rochester, NY | 2024 |
| LOCUS IMPACT FUND | $10,759,380 | S40 | published | | Christiansbrg, VA | 2024 |
| PACIFIC WEST ASSOCIATION OF REALTORS INC | $10,432,130 | S40 | published | | Anaheim, CA | 2024 |
| YOGA ALLIANCE | $10,343,431 | S40 | published | | Arlington, VA | 2024 |
| AUTOMATION ALLEY | $9,944,782 | S40 | published | | Troy, MI | 2024 |
| COALITION FOR COMMUNITY SOLAR ACCESS | $9,245,770 | S40 | published | | Washington, DC | 2024 |
| US-ASEAN BUSINESS COUNCIL INC | $8,943,979 | S40 | published | | Washington, DC | 2024 |
| RISC-V INTERNATIONAL ASSOCIATION VEREIN | $8,584,520 | S40 | published | | Wilmington, DE | 2024 |
| OUT-OF-HOME ADVERTISING ASSOCIATION OF AMERICA | $8,516,753 | S40 | inferred 0.75 | girls boys: boys girls clubs | Washington, DC | 2024 |
| WV REGIONAL TECHNOLOGY PARK CORP | $8,438,326 | S40 | published | | S Charleston, WV | 2011 |
| NATIVE AMERICAN DEVELOPMENT CORPORATION | $8,372,908 | S40 | published | abuse: substance | Billings, MT | 2024 |
| ASSOCIATION FOR HEALTH CENTER AFFILIATED HEALTH PLANS | $8,012,471 | S40 | published | | Washington, DC | 2024 |
| GREENPOINT MANUFACTURING AND DESIGN CENTER LOCAL DEVELOPMENT CORP GMDC | $7,917,281 | S40 | published | | Brooklyn, NY | 2025 |
| INTERNATIONAL AUTOMOTIVE OVERSIGHT BUREAU | $7,894,532 | S40 | published | | Southfield, MI | 2024 |
| CRYPTO OPEN PATENT ALLIANCE | $7,830,791 | S40 | published | | Palo Alto, CA | 2024 |
| LTC REINSURANCE PCC | $7,801,774 | S40 | published | | Washington, DC | 2025 |
| PHILADELPHIA GREEN CAPITAL CORP | $7,728,755 | S40 | published | | Philadelphia, PA | 2024 |
| WOOD INDUSTRY ASSOCIATION | $7,714,974 | S40 | published | | Forest Hill, MD | 2024 |
| WORKING FOREST INITIATIVE | $7,625,000 | S40 | published | | Savannah, GA | 2024 |
| CCDC COMMON AREA ASSOCIATION | $7,427,597 | S40 | published | | Washington, DC | 2024 |
| SOUTHEAST COASTAL OCEAN OBSERVING REGIONAL ASSOCIATION | $7,375,802 | S40 | published | science: research education | Charleston, SC | 2024 |
| WILLIAM A LAWSON INSTITUTE FOR PEACE & PROSPERITY | $7,372,947 | S40 | published | men: issues | Houston, TX | 2024 |
| CONNSTEP INC | $7,278,492 | S40 | published | | Hartford, CT | 2024 |
| BLOCKCHAIN ASSOCIATION | $7,128,656 | S40 | published | | Washington, DC | 2024 |
| DELAWARE INNOVATION SPACE INC | $7,062,650 | S40 | published | | Wilmington, DE | 2025 |
| ALTOONA-BLAIR COUNTY DEVELOPMENT CORPORATION | $6,733,762 | S40 | published | | Altoona, PA | 2025 |
| SCIENCE PARK DEVELOPMENT CORPORATION | $6,688,368 | S40 | published | | New Haven, CT | 2024 |
| NATIONAL INSTITUTE OF MINORITY ECONOMIC DEVELOPMENT | $6,532,015 | S40 | inferred 0.50 | women: issues | Durham, NC | 2024 |
| NEW VILLAGE CORPORATION | $5,943,070 | S40 | published | | Cleveland, OH | 2024 |
| AUTO-ISAC INC | $5,898,599 | S40 | published | | Washington, DC | 2024 |
| BETTER BUSINESS BUREAU OF GREATER ST LOUIS INC | $5,871,172 | S40 | inferred 0.80 | | Saint Louis, MO | 2024 |
| BUSINESSES UNITED IN INVESTING LENDING AND DEVELOPMENT | $5,834,976 | S40 | published | | Redwood City, CA | 2024 |
| BETTER BUSINESS BUREAU OF PALM BEACH COUNTY INC | $5,817,084 | S40 | inferred 0.80 | | West Palm Bch, FL | 2025 |
| AVIATION INFORMATION SHARING AND ANALYSIS CENTER INC | $5,668,230 | S40 | published | | Annapolis, MD | 2025 |
| TRUSTWORTHY ACCOUNTABILITY GROUP INC | $5,421,027 | S40 | published | | Washington, DC | 2025 |
| NEIGHBORHOOD COMMUNITY DEVELOPMENT FUND | $5,316,023 | S40 | published | | Pittsburgh, PA | 2024 |
| FCLT CO | $5,152,881 | S40 | published | | Boston, MA | 2024 |
| ALL RAISE | $5,103,761 | S40 | published | space: for women | West Hollywood, CA | 2024 |
| PRIMEGLOBAL INC | $5,073,007 | S40 | published | | Duluth, GA | 2024 |
| INTERNATIONAL DOOR ASSOCIATION INC | $5,053,862 | S40 | inferred 0.80 | | Washington, DC | 2024 |
| BATTLE CREEK UNLIMITED INC | $4,959,749 | S40 | published | science: research education | Battle Creek, MI | 2024 |
| MEDICAID HEALTH PLANS OF AMERICA | $4,934,335 | S40 | published | | Washington, DC | 2024 |
| BUILDING CONTRACTORS ASSOCIATION INC | $4,847,864 | S40 | inferred 0.55 | | New York, NY | 2024 |
| MBCA INC | $4,804,700 | S40 | published | | Miramar, FL | 2025 |
| FINANCIAL & INSURANCE CONFERENCE PROFESSIONALS | $4,804,204 | S40 | published | | Chicago, IL | 2025 |
| AMERICAN ACADEMY OF EMERGENCY MEDICINE INC | $4,704,892 | S40 | published | science: research education | Milwaukee, WI | 2025 |
| OKLAHOMA ALLIANCE FOR MANUFACTURING EXCELLENCE INC | $4,697,902 | S40 | published | | Tulsa, OK | 2024 |
| OREGON FOREST INDUSTRIES COUNCIL | $4,687,612 | S40 | published | science: research education | Salem, OR | 2024 |
| NOMA IMPROVEMENT ASSOCIATION | $4,559,404 | S40 | published | | Washington, DC | 2024 |
| BETTER BUSINESS BUREAU OF WEST FLORIDA INC | $4,448,715 | S40 | inferred 0.85 | | Clearwater, FL | 2025 |
| OPEN AVENUES FOUNDATION | $4,405,051 | S40 | published | | Boston, MA | 2024 |
| THE INTERNATIONAL JOINT COMMISSION ON ALLIED HEALTH PERSONNEL IN OPHT | $4,394,686 | S40 | published | | Woodbury, MN | 2024 |
| DELAWARE COMPENSATION RATING BUREAU | $4,384,093 | S40 | inferred 0.50 | | Philadelphia, PA | 2024 |
| NATIONAL RENTAL HOME COUNCIL | $4,375,683 | S40 | published | | Dallas, TX | 2024 |
| DIRECTTRUST ORG INC | $4,239,162 | S40 | published | | Washington, DC | 2025 |
| COMMONWELL HEALTH ALLIANCE INC | $4,225,791 | S40 | published | | Boston, MA | 2024 |