Patents
US 8975016 B2: Methods for direct fluorescent antibody virus detection in liquids
- Granted
- 2015-03-10
- Filed
- 2011-06-22 application 13166228
- Type
- Utility · 24 claims
- Elsewhere
- Google Patents ↗ · USPTO patent document ↗ · USPTO Patent Center record ↗
Abstract
The present invention describes a liquid direct fluorescence antibody assay that is rapid and sensitive to detect respiratory virus in infected cells. The assay includes centrifugation of the specimen, incubation of sample and reagents in solution, and detection of the absence or presence of respiratory virus. Sapogenin is used as a detergent to permeabilize the cells for entry of the monoclonal antibodies to react with intracellular antigens. The cells are stained with fluorescently labeled monoclonal antibodies against the viral antigens along with a background stain and a fluorescent nuclear stain. This counter staining decreases background and allows co-localization of antigen and nuclear structures for enhanced detection.
Source: PVGPATDIS, file of 2026-04-10 (ipg150310.xml).
Assignees
| Assignee | Type | Place | Also on furp.org | Source |
|---|---|---|---|---|
| Diagnostic Hybrids, Inc. 13 patents | US company or organization | Athens, OH | DIAGNOSTIC HYBRIDS, INC. State business registration EXACT high confidence: identical full name and state | PVGPATDIS, file of 2026-04-10 |
Inventors
| Inventor | Place | Patents | Person page | Source |
|---|---|---|---|---|
| David R. Scholl | Athens, OH | 9 | Person page FUZZY medium confidence | PVGPATDIS, file of 2026-04-10 |
| James L. Brown | Athens, OH | 12 | PVGPATDIS, file of 2026-04-10 | |
| Joseph A. Jollick, Jr. | Athens, OH | 3 | PVGPATDIS, file of 2026-04-10 | |
| Ronald Lollar | Athens, OH | 3 | PVGPATDIS, file of 2026-04-10 |
CPC categories
| Subclass | Category | First group | Source |
|---|---|---|---|
| G01N | Investigating or analysing materials by determining their chemical or physical properties | G01N33/56983 | PVGPATDIS, file of 2026-04-10 |
Cooperative Patent Classification, as currently assigned (one row per subclass).
Assignments and security interests
| Recorded | What | From | To | Reel/frame | Source |
|---|---|---|---|---|---|
| 2025-08-22 | Release of security interest Release (reel 060220 / frame 0711) | BANK OF AMERICA, N.A. signed 2025-08-21 | QUIDEL CORPORATION SAN DIEGO, CA BIOHELIX CORPORATION BEVERLY, MA DIAGNOSTIC HYBRIDS, INC. ATHENS, OH QUIDEL CARDIOVASCULAR INC. SAN DIEGO, CA ORTHO-CLINICAL DIAGNOSTICS, INC. RARITAN, NJ CRIMSON U.S. ASSETS LLC RARITAN, NJ CRIMSON INTERNATIONAL ASSETS LLC RARITAN, NJ MICRO TYPING SYSTEMS, INC. POMPANO BEACH, FL | 72577/536 | PASDL, file of 2026-10-15 |
| 2025-08-22 | Security interest Security interest Security agreement | CRIMSON INTERNATIONAL ASSETS LLC signed 2025-08-21 MICRO TYPING SYSTEMS, INC. signed 2025-08-21 ORTHO-CLINICAL DIAGNOSTICS, INC. signed 2025-08-21 QUIDEL CARDIOVASCULAR INC. signed 2025-08-21 | BANK OF AMERICA, N.A., AS ADMINISTRATIVE AGENT CHARLOTTE, NC | 72526/643 | PASDL, file of 2026-10-15 |
| 2024-08-15 | Assignment Assignment of assignor's interest | DIAGNOSTICS HYBRIDS, INC. signed 2024-08-12 | ORTHO-CLINICAL DIAGNOSTICS, INC. RARITAN, NJ | 68657/748 | ECORSEXC, file of 2026-09-25 |
| 2022-05-31 | Security interest Security interest Security agreement | QUIDEL CORPORATION signed 2022-05-27 BIOHELIX CORPORATION signed 2022-05-27 DIAGNOSTIC HYBRIDS, INC. signed 2022-05-27 QUIDEL CARDIOVASCULAR INC. signed 2022-05-27 ORTHO-CLINICAL DIAGNOSTICS, INC. signed 2022-05-27 CRIMSON U.S. ASSETS LLC signed 2022-05-27 CRIMSON INTERNATIONAL ASSETS LLC signed 2022-05-27 MICRO TYPING SYSTEMS, INC. signed 2022-05-27 | BANK OF AMERICA, N.A. CHARLOTTE, NC | 60220/711 | PASDL, file of 2026-10-15 |
| 2022-05-31 | Release of security interest Release of security interest | BANK OF AMERICA, N.A. signed 2022-05-27 | QUIDEL CORPORATION SAN DIEGO, CA | 60220/649 | PASDL, file of 2026-10-15 |
| 2017-10-06 | Security interest Security interest Security interest | QUIDEL CORPORATION signed 2017-10-06 | BANK OF AMERICA, N.A., AS ADMINISTRATIVE AGENT KIRKLAND, WA | 44141/686 | PASDL, file of 2026-10-15 |
| 2017-01-12 | Name change Change of address | DIAGNOSTIC HYBRIDS, INC. signed 2016-10-17 | DIAGNOSTIC HYBRIDS, INC. ATHENS, OH | 41351/846 | PASDL, file of 2026-10-15 |
| 2016-12-01 | Release of security interest Notice of release of grant of patent security interest | BANK OF AMERICA, N.A., AS AGENT signed 2016-12-01 | DIAGNOSTIC HYBRIDS, INC. ATHENS, OH | 40788/523 | ECORSEXC, file of 2026-09-25 |
| 2012-09-18 | Security interest Security interest Grant of patent security interest | DIAGNOSTIC HYBRIDS, INC. signed 2012-08-10 | BANK OF AMERICA, N.A., AS ADMINISTRATIVE AGENT SEATTLE, WA | 29002/803 | ECORSEXC, file of 2026-09-25 |
| 2011-06-22 | Assignment Assignment of assignor's interest | SCHOLL, DAVID R. signed 2009-06-02 JOLLICK, JOSEPH A., JR. signed 2009-06-03 BROWN, JAMES L. signed 2009-06-05 LOLLAR, RONALD signed 2009-06-08 | DIAGNOSTIC HYBRIDS, INC. ATHENS, OH | 26483/813 | ECORSEXC, file of 2026-09-25 |
Documents recorded with the USPTO Assignment Recordation Branch that list this patent. A security interest is a lender’s claim on the patent as collateral; a release ends it.
USPTO bulk data: PatentsView granted patents, disambiguated (PVGPATDIS) for patents, assignees, inventors, places and CPC categories; the USPTO Patent Assignment Dataset (ECORSEXC) for assignments and security interests. Patents filed in the last 20 years. No claims or full text. Links to other records are by name (and state): EXACT means the identical full name and state; FUZZY means a looser name match, with the confidence shown.