Patents
US 12723264 B2: S. pyogenes CAS9 mutant genes and polypeptides encoded by same
- Granted
- 2026-09-01
- Filed
- 2024-01-20 application 18418248
- Type
- Utility · 17 claims
- Elsewhere
- Google Patents ↗ · USPTO patent document ↗ · USPTO Patent Center record ↗
Abstract
This invention pertains to isolated mutant Cas9 nucleic acids and proteins for use in CRISPR/Cas endonuclease systems and their methods of use. In particular, the invention pertains to an isolated mutant Cas9 protein, wherein the isolated mutant Cas9 protein is active in a CRISPR/Cas endonuclease system, wherein the CRISPR/Cas endonuclease system displays a lower ratio of reduced off-target editing activity to on-target editing activity for at least one target site relative to the corresponding ratio of reduced off-site editing activity to on-target editing activity of a wild-type CRISPR/Cas endonuclease system having a WT-Cas9 protein of SEQ ID NO:5.
Source: PTBLXML, file of 2026-09-01 (ipgb20260901.xml).
Assignees
| Assignee | Type | Place | Also on furp.org | Source |
|---|---|---|---|---|
| Integrated DNA Technologies, Inc. 103 patents | US company or organization | Coralville, IA | INTEGRATED DNA TECHNOLOGIES, INC. State business registration EXACT high confidence: identical full name and state | PTBLXML, file of 2026-09-01 |
Inventors
| Inventor | Place | Patents | Person page | Source |
|---|---|---|---|---|
| Christopher Anthony Vakulskas | North Liberty, IA | 22 | PTBLXML, file of 2026-09-01 | |
| Nicole Mary Bode | Oxford, IA | 5 | PTBLXML, file of 2026-09-01 | |
| Michael Allen Collingwood | North Liberty, IA | 19 | PTBLXML, file of 2026-09-01 | |
| Garrett Richard Rettig | Coralville, IA | 15 | PTBLXML, file of 2026-09-01 | |
| Mark Aaron Behlke | Coralville, IA | 74 | PTBLXML, file of 2026-09-01 |
CPC categories
| Subclass | Category | First group | Source |
|---|---|---|---|
| C12N | Microorganisms or enzymes; compositions thereof; propagating, preserving, or maintaining microorganisms; mutation or genetic engineering; culture media | C12N15/87 | PTBLXML, file of 2026-09-01 |
| C12Q | Measuring or testing processes involving enzymes, nucleic acids or microorganisms ; compositions or test papers therefor; processes of preparing such compositions; condition-responsive control in microbiological or enzymological processes | C12Q1/6897 | PTBLXML, file of 2026-09-01 |
| C12P | Fermentation or enzyme-using processes to synthesise a desired chemical compound or composition or to separate optical isomers from a racemic mixture | C12P19/34 | PTBLXML, file of 2026-09-01 |
Cooperative Patent Classification, as currently assigned (one row per subclass).
Assignments and security interests
No recorded assignments or security interests on file for this patent.
USPTO bulk data: PatentsView granted patents, disambiguated (PVGPATDIS) for patents, assignees, inventors, places and CPC categories; the USPTO Patent Assignment Dataset (ECORSEXC) for assignments and security interests. Patents filed in the last 20 years. No claims or full text. Links to other records are by name (and state): EXACT means the identical full name and state; FUZZY means a looser name match, with the confidence shown.