Patents
US 12644133 B2: Methods and compositions to increase somatic cell nuclear transfer (SCNT) efficiency by removing histone H3-lysine trimethylation
- Granted
- 2026-06-02
- Filed
- 2022-06-14 application 17840348
- Type
- Utility · 12 claims
- Elsewhere
- Google Patents ↗ · USPTO patent document ↗ · USPTO Patent Center record ↗
Abstract
The present invention provides methods and compostions to improve the efficiency of somatic cell nuclear transfer (SCNT) and the consequent production of nuclear transfer ESC (ntESC) and transgenic cells and/or non-human animals. More specifically, the present invention relates to the discovery that trimethylation of Histone H3-Lysine 9 (H3K9me3) in reprogramming resistant regions (RRRs) in the nuclear genetic material of donor somatic cells prevents efficient somatic cell nuclear reprogramming or SCNT. The present invention provide methods and compositions to decrease H3K9me3 in methods to improve efficacy of SCNT by exogenous or overexpression of the demethylase Kdm4 family and/or inhibiting methylation of H3K9me3 by inhibiting the histone methyltransferases Suv39h1 and/or Suv39h2.
Source: PTBLXML, file of 2026-06-02 (ipgb20260602.xml).
Assignees
| Assignee | Type | Place | Also on furp.org | Source |
|---|---|---|---|---|
| The Children's Medical Center Corporation 717 patents | US company or organization | Boston, MA | THE CHILDREN'S MEDICAL CENTER CORPORATION State business registration FUZZY medium confidence: same name (legal endings aside) and state | PTBLXML, file of 2026-06-02 |
Inventors
| Inventor | Place | Patents | Person page | Source |
|---|---|---|---|---|
| Yi Zhang | Boston, MA | 86 | PTBLXML, file of 2026-06-02 | |
| Shogo Matoba | Tsukuba, JP | 3 | PTBLXML, file of 2026-06-02 |
CPC categories
| Subclass | Category | First group | Source |
|---|---|---|---|
| C12N | Microorganisms or enzymes; compositions thereof; propagating, preserving, or maintaining microorganisms; mutation or genetic engineering; culture media | C12N15/877 | PTBLXML, file of 2026-06-02 |
| A01K | Animal husbandry; aviculture; apiculture; pisciculture; fishing; rearing or breeding animals, not otherwise provided for; new breeds of animals | A01K67/0273 | PTBLXML, file of 2026-06-02 |
| C12Y | Enzymes | C12Y201/01043 | PTBLXML, file of 2026-06-02 |
Cooperative Patent Classification, as currently assigned (one row per subclass).
Assignments and security interests
| Recorded | What | From | To | Reel/frame | Source |
|---|---|---|---|---|---|
| 2025-03-13 | Assignment Assignment of assignor's interest | ZHANG, YI signed 2017-05-04 MATOBA, SHOGO signed 2017-05-08 | CHILDREN'S MEDICAL CENTER CORPORATION BOSTON, MA | 70496/443 | PASDL, file of 2026-10-15 |
Documents recorded with the USPTO Assignment Recordation Branch that list this patent. A security interest is a lender’s claim on the patent as collateral; a release ends it.
USPTO bulk data: PatentsView granted patents, disambiguated (PVGPATDIS) for patents, assignees, inventors, places and CPC categories; the USPTO Patent Assignment Dataset (ECORSEXC) for assignments and security interests. Patents filed in the last 20 years. No claims or full text. Links to other records are by name (and state): EXACT means the identical full name and state; FUZZY means a looser name match, with the confidence shown.