Patents
US 12365937 B2: Asymmetric targeted amplification methods
- Granted
- 2025-07-22
- Filed
- 2021-02-23 application 17182937
- Type
- Utility · 20 claims
- Elsewhere
- Google Patents ↗ · USPTO patent document ↗ · USPTO Patent Center record ↗
Abstract
The present disclosure provides kits and methods for a targeted NGS library workflow comprising a multiplexed PCR for target amplification followed by an indexing PCR for incorporation of NGS adapter sequences. This workflow enables overlapping primer pairs in a single tube for contiguous coverage over target regions, while simultaneously preventing amplification of both primer dimers and undesirable mini-amplicons that result from overlapping primer pairs.
Source: PVGPATDIS, file of 2026-04-10 (ipg250722.xml).
Assignees
| Assignee | Type | Place | Also on furp.org | Source |
|---|---|---|---|---|
| INTEGRATED DNA TECHNOLOGIES, INC. 103 patents | US company or organization | Coralville, IA | INTEGRATED DNA TECHNOLOGIES, INC. State business registration EXACT high confidence: identical full name and state | PVGPATDIS, file of 2026-04-10 |
Inventors
| Inventor | Place | Patents | Person page | Source |
|---|---|---|---|---|
| Ashley M. WOOD | Ann Arbor, MI | 1 | PVGPATDIS, file of 2026-04-10 | |
| Jordan Rosefigura | Ann Arbor, MI | 1 | PVGPATDIS, file of 2026-04-10 | |
| Vladimir Makarov | Ann Arbor, MI | 38 | PVGPATDIS, file of 2026-04-10 |
CPC categories
| Subclass | Category | First group | Source |
|---|---|---|---|
| C12N | Microorganisms or enzymes; compositions thereof; propagating, preserving, or maintaining microorganisms; mutation or genetic engineering; culture media | C12N15/1093 | PVGPATDIS, file of 2026-04-10 |
| C12Q | Measuring or testing processes involving enzymes, nucleic acids or microorganisms ; compositions or test papers therefor; processes of preparing such compositions; condition-responsive control in microbiological or enzymological processes | C12Q1/6848 | PVGPATDIS, file of 2026-04-10 |
Cooperative Patent Classification, as currently assigned (one row per subclass).
Assignments and security interests
| Recorded | What | From | To | Reel/frame | Source |
|---|---|---|---|---|---|
| 2025-07-09 | Assignment Assignment of assignor's interest | WOOD, ASHLEY M. signed 2020-12-11 ROSEFIGURA, JORDAN signed 2020-12-11 MAKAROV, VLADIMIR signed 2020-12-14 | INTEGRATED DNA TECHNOLOGIES, INC. CORALVILLE, IA | 71640/242 | PASDL, file of 2026-10-18 |
| 2022-04-29 | Assignment Assignment of assignor's interest | ROSEFIGURA, JORDAN signed 2020-12-11 WOOD, ASHLEY M. signed 2020-12-11 MAKAROV, VLADIMIR signed 2020-12-14 | SWIFT BIOSCIENCES, INC. ANN ARBOR, MI | 59770/273 | ECORSEXC, file of 2026-09-25 |
| 2022-04-15 | Merger Merger | SWIFT BIOSCIENCES, INC. signed 2022-03-18 | INTEGRATED DNA TECHNOLOGIES, INC. CORALVILLE, IA | 59606/848 | PASDL, file of 2026-10-15 |
Documents recorded with the USPTO Assignment Recordation Branch that list this patent. A security interest is a lender’s claim on the patent as collateral; a release ends it.
USPTO bulk data: PatentsView granted patents, disambiguated (PVGPATDIS) for patents, assignees, inventors, places and CPC categories; the USPTO Patent Assignment Dataset (ECORSEXC) for assignments and security interests. Patents filed in the last 20 years. No claims or full text. Links to other records are by name (and state): EXACT means the identical full name and state; FUZZY means a looser name match, with the confidence shown.