Patents
US 12297435 B2: Mutations in Streptococcus pyogenes Cas9 discovered by broad scanning mutagenesis demonstrate enhancement of DNA cleavage activity
- Granted
- 2025-05-13
- Filed
- 2022-07-05 application 17857262
- Type
- Utility · 14 claims
- Elsewhere
- Google Patents ↗ · USPTO patent document ↗ · USPTO Patent Center record ↗
Abstract
This invention pertains to mutant Cas9 nucleic acids and proteins for use in CRISPR/Cas endonuclease systems, and their methods of use. In particular, the invention pertains to an isolated mutant Cas9 protein, wherein the isolated mutant Cas9 protein is active in a CRISPR/Cas endonuclease system, wherein the CRISPR/Cas endonuclease system displays increased on-target editing activity relative to a wild-type CRISPR/Cas endonuclease system. The invention also includes isolated nucleic acids encoding mutant Cas9 proteins, ribonucleoprotein complexes and CRISPR/Cas endonuclease systems having mutant Cas9 proteins that display increased on-target editing activity relative to a wild-type CRISPR/Cas endonuclease system.
Source: PVGPATDIS, file of 2026-04-10 (ipg250513.xml).
Assignees
| Assignee | Type | Place | Also on furp.org | Source |
|---|---|---|---|---|
| INTEGRATED DNA TECHNOLOGIES, INC. 103 patents | US company or organization | Coralville, IA | INTEGRATED DNA TECHNOLOGIES, INC. State business registration EXACT high confidence: identical full name and state | PVGPATDIS, file of 2026-04-10 |
Inventors
| Inventor | Place | Patents | Person page | Source |
|---|---|---|---|---|
| Nathaniel Hunter Roberts | Iowa City, IA | 1 | PVGPATDIS, file of 2026-04-10 | |
| Liyang Zhang | North Liberty, IA | 47 | PVGPATDIS, file of 2026-04-10 | |
| Christopher Anthony Vakulskas | North Liberty, IA | 22 | PVGPATDIS, file of 2026-04-10 |
CPC categories
| Subclass | Category | First group | Source |
|---|---|---|---|
| C12N | Microorganisms or enzymes; compositions thereof; propagating, preserving, or maintaining microorganisms; mutation or genetic engineering; culture media | C12N15/52 | PVGPATDIS, file of 2026-04-10 |
Cooperative Patent Classification, as currently assigned (one row per subclass).
Assignments and security interests
| Recorded | What | From | To | Reel/frame | Source |
|---|---|---|---|---|---|
| 2025-04-14 | Assignment Assignment of assignor's interest | VAKULSKAS, CHRISTOPHER ANTHONY signed 2025-03-20 ROBERTS, NATHANIEL HUNTER signed 2025-04-08 ZHANG, LIYANG signed 2025-04-11 | INTEGRATED DNA TECHNOLOGIES, INC. CORALVILLE, IA | 70832/956 | ECORSEXC, file of 2026-09-25 |
Documents recorded with the USPTO Assignment Recordation Branch that list this patent. A security interest is a lender’s claim on the patent as collateral; a release ends it.
USPTO bulk data: PatentsView granted patents, disambiguated (PVGPATDIS) for patents, assignees, inventors, places and CPC categories; the USPTO Patent Assignment Dataset (ECORSEXC) for assignments and security interests. Patents filed in the last 20 years. No claims or full text. Links to other records are by name (and state): EXACT means the identical full name and state; FUZZY means a looser name match, with the confidence shown.