Patents
US 12024742 B2: RNASE H2 mutants that reduce primer dimers and off-target amplification in RHPCR-based amplicon sequencing with high-fidelity DNA polymerases
- Granted
- 2024-07-02
- Filed
- 2021-12-22 application 17558994
- Type
- Utility · 11 claims
- Elsewhere
- Google Patents ↗ · USPTO patent document ↗ · USPTO Patent Center record ↗
Abstract
The present invention pertains to hybrid RNase H2 proteins that include fragments of amino acid sequences from Pyrococcus abyssi (P.a.), Thermococcus kodakarensis (T.kod), and Pyrococcus furiosus organisms, as well as methods of using the same to improve mismatch discrimination and activity in a high-fidelity DNA polymerase buffer.
Source: PVGPATDIS, file of 2026-04-10 (ipg240702.xml).
Assignees
| Assignee | Type | Place | Also on furp.org | Source |
|---|---|---|---|---|
| INTEGRATED DNA TECHNOLOGIES, INC. 103 patents | US company or organization | Coralville, IA | INTEGRATED DNA TECHNOLOGIES, INC. State business registration EXACT high confidence: identical full name and state | PVGPATDIS, file of 2026-04-10 |
Inventors
| Inventor | Place | Patents | Person page | Source |
|---|---|---|---|---|
| Joseph Dobosy | Coralville, IA | 12 | PVGPATDIS, file of 2026-04-10 | |
| John Froehlig, Jr. | North Liberty, IA | 1 | PVGPATDIS, file of 2026-04-10 | |
| Katherine Perschbacher | Coralville, IA | 1 | PVGPATDIS, file of 2026-04-10 | |
| Kristin Beltz | Cedar Rapids, IA | 5 | PVGPATDIS, file of 2026-04-10 | |
| Scott Rose | Coralville, IA | 16 | PVGPATDIS, file of 2026-04-10 | |
| Mark Aaron Behlke | Coralville, IA | 74 | PVGPATDIS, file of 2026-04-10 |
CPC categories
| Subclass | Category | First group | Source |
|---|---|---|---|
| C12N | Microorganisms or enzymes; compositions thereof; propagating, preserving, or maintaining microorganisms; mutation or genetic engineering; culture media | C12N15/62 | PVGPATDIS, file of 2026-04-10 |
| C12Q | Measuring or testing processes involving enzymes, nucleic acids or microorganisms ; compositions or test papers therefor; processes of preparing such compositions; condition-responsive control in microbiological or enzymological processes | C12Q1/6848 | PVGPATDIS, file of 2026-04-10 |
| C12Y | Enzymes | C12Y301/26004 | PVGPATDIS, file of 2026-04-10 |
| C07K | Peptides | C07K2319/00 | PVGPATDIS, file of 2026-04-10 |
| C12R | Indexing scheme associated with subclasses c12c - c12q, relating to microorganisms | C12R2001/01 | PVGPATDIS, file of 2026-04-10 |
Cooperative Patent Classification, as currently assigned (one row per subclass).
Assignments and security interests
| Recorded | What | From | To | Reel/frame | Source |
|---|---|---|---|---|---|
| 2023-06-20 | Assignment Assignment of assignor's interest | BEHLKE, MARK AARON signed 2023-05-31 DOBOSY, JOSEPH signed 2023-05-31 FROEHLIG, JOHN signed 2023-05-31 BELTZ, KRISTIN signed 2023-06-06 PERSCHBACHER, KATHERINE signed 2023-06-06 ROSE, SCOTT signed 2023-06-06 | INTEGRATED DNA TECHNOLOGIES, INC. CORALVILLE, IA | 63997/53 | ECORSEXC, file of 2026-09-25 |
Documents recorded with the USPTO Assignment Recordation Branch that list this patent. A security interest is a lender’s claim on the patent as collateral; a release ends it.
USPTO bulk data: PatentsView granted patents, disambiguated (PVGPATDIS) for patents, assignees, inventors, places and CPC categories; the USPTO Patent Assignment Dataset (ECORSEXC) for assignments and security interests. Patents filed in the last 20 years. No claims or full text. Links to other records are by name (and state): EXACT means the identical full name and state; FUZZY means a looser name match, with the confidence shown.