Patents
US 10865436 B2: Microfluidic apparatus, method, and applications
- Granted
- 2020-12-15
- Filed
- 2014-09-05 application 14478012
- Type
- Utility · 2 claims
- Elsewhere
- Google Patents ↗ · USPTO patent document ↗ · USPTO Patent Center record ↗
Abstract
A microfluidic apparatus, method, and associated applications utilize and apply to a formalin-fixed paraffin-embedded (FFPE) tissue sample and performing a liquid-liquid extraction to remove the paraffin from the tissue sample prior to a nucleic acid purification step. A microfluidic device includes a dedicated liquid-liquid extraction process vessel, a nucleic acid purification process component, and a nucleic acid amplification reactor. A liquid-liquid extraction and nucleic acid purification kit includes a microfluidic device capable of performing both a liquid-liquid extraction process and a nucleic acid purification process, including a dedicated liquid-liquid extraction process vessel, an immiscible liquid or a precursor phase thereof disposed in the vessel, a nucleic acid purification process component, a nucleic acid amplification reactor fluidically, and a supply of reagents suitable to enable the liquid-liquid extraction process and the nucleic acid purification process.
Source: PVGPATDIS, file of 2026-04-10 (ipg201215.xml).
Assignees
| Assignee | Type | Place | Also on furp.org | Source |
|---|---|---|---|---|
| Rheonix, Inc. 38 patents | US company or organization | Ithaca, NY | RHEONIX INC Form D issuer EXACT high confidence: identical full name and state | PVGPATDIS, file of 2026-04-10 |
Inventors
| Inventor | Place | Patents | Person page | Source |
|---|---|---|---|---|
| Peng Zhou | Newtown, PA | 52 | PVGPATDIS, file of 2026-04-10 |
CPC categories
| Subclass | Category | First group | Source |
|---|---|---|---|
| C12Q | Measuring or testing processes involving enzymes, nucleic acids or microorganisms ; compositions or test papers therefor; processes of preparing such compositions; condition-responsive control in microbiological or enzymological processes | C12Q1/6806 | PVGPATDIS, file of 2026-04-10 |
| B01L | Chemical or physical laboratory apparatus for general use | B01L3/502715 | PVGPATDIS, file of 2026-04-10 |
| C07H | Sugars; derivatives thereof; nucleosides; nucleotides; nucleic acids | C07H1/08 | PVGPATDIS, file of 2026-04-10 |
| C12N | Microorganisms or enzymes; compositions thereof; propagating, preserving, or maintaining microorganisms; mutation or genetic engineering; culture media | C12N15/1003 | PVGPATDIS, file of 2026-04-10 |
Cooperative Patent Classification, as currently assigned (one row per subclass).
Assignments and security interests
| Recorded | What | From | To | Reel/frame | Source |
|---|---|---|---|---|---|
| 2021-05-17 | Security interest Security interest Security interest | RHEONIX, INC. signed 2021-05-17 | GRASSHOPPER BANK, N.A. NEW YORK, NY | 56254/74 | ECORSEXC, file of 2026-09-25 |
| 2014-09-05 | Assignment Assignment of assignor's interest | ZHOU, PENG signed 2014-09-02 | RHEONIX, INC. ITHACA, NY | 33674/612 | ECORSEXC, file of 2026-09-25 |
Documents recorded with the USPTO Assignment Recordation Branch that list this patent. A security interest is a lender’s claim on the patent as collateral; a release ends it.
USPTO bulk data: PatentsView granted patents, disambiguated (PVGPATDIS) for patents, assignees, inventors, places and CPC categories; the USPTO Patent Assignment Dataset (ECORSEXC) for assignments and security interests. Patents filed in the last 20 years. No claims or full text. Links to other records are by name (and state): EXACT means the identical full name and state; FUZZY means a looser name match, with the confidence shown.