Patents
US 10329605 B2: Method to increase sensitivity of detection of low-occurrence mutations
- Granted
- 2019-06-25
- Filed
- 2016-04-20 application 15134302
- Type
- Utility · 10 claims
- Elsewhere
- Google Patents ↗ · USPTO patent document ↗ · USPTO Patent Center record ↗
Abstract
A method for detecting a low-occurrence mutation in isolated DNA adds a blocking probe to reagents during amplification of the isolated DNA. The blocking probe is an oligonucleotide complementary to wild-type DNA corresponding to the sample. The blocking probe spans a site of a suspected mutation within a region of interest in the isolated DNA. After amplification, fragments of the amplified DNA is sequenced using next generating sequencing and an output is generated to display the sequenced fragments. In some embodiments, the blocking probe is locked nucleic acid (LNA).
Source: PVGPATDIS, file of 2026-04-10 (ipg190625.xml).
Assignees
| Assignee | Type | Place | Also on furp.org | Source |
|---|---|---|---|---|
| NeoGenomics Laboratories, Inc. 6 patents | Foreign company or organization | Fort Myers, FL | PVGPATDIS, file of 2026-04-10 |
Inventors
| Inventor | Place | Patents | Person page | Source |
|---|---|---|---|---|
| Maher Albitar | Valley Center, CA | 53 | PVGPATDIS, file of 2026-04-10 |
CPC categories
| Subclass | Category | First group | Source |
|---|---|---|---|
| C12Q | Measuring or testing processes involving enzymes, nucleic acids or microorganisms ; compositions or test papers therefor; processes of preparing such compositions; condition-responsive control in microbiological or enzymological processes | C12Q1/6858 | PVGPATDIS, file of 2026-04-10 |
Cooperative Patent Classification, as currently assigned (one row per subclass).
Assignments and security interests
| Recorded | What | From | To | Reel/frame | Source |
|---|---|---|---|---|---|
| 2020-05-11 | Release of security interest Release of security interest | PNC BANK, NATIONAL ASSOCIATION, AS ADMINISTRATIVE AGENT signed 2020-05-04 | NEOGENOMICS LABORATORIES, INC. NAPLES, FL | 52625/295 | ECORSEXC, file of 2026-09-25 |
| 2019-06-28 | Release of security interest Termination and release of security interest in patents | REGIONS BANK, AS COLLATERAL AGENT signed 2019-06-27 | NEOGENOMICS LABORATORIES, INC. FORT MYERS, FL | 49629/235 | ECORSEXC, file of 2026-09-25 |
| 2019-06-28 | Security interest Security interest Notice of grant of security interest in patents | NEOGENOMICS LABORATORIES, INC. signed 2019-06-27 | PNC BANK, NATIONAL ASSOCIATION, AS ADMINISTRATIVE AGENT PITTSBURGH, PA | 49624/512 | ECORSEXC, file of 2026-09-25 |
| 2017-04-13 | Assignment Assignment of assignor's interest | ALBITAR, MAHER signed 2017-04-11 | NEOGENOMICS LABORATORIES, INC. FORT MYERS, FL | 42004/858 | ECORSEXC, file of 2026-09-25 |
| 2016-12-23 | Security interest Security interest Notice of grant of security interest in patents | NEOGENOMICS LABORATORIES, INC. signed 2016-12-22 | REGIONS BANK, AS COLLATERAL AGENT ATLANTA, GA | 41182/717 | ECORSEXC, file of 2026-09-25 |
Documents recorded with the USPTO Assignment Recordation Branch that list this patent. A security interest is a lender’s claim on the patent as collateral; a release ends it.
USPTO bulk data: PatentsView granted patents, disambiguated (PVGPATDIS) for patents, assignees, inventors, places and CPC categories; the USPTO Patent Assignment Dataset (ECORSEXC) for assignments and security interests. Patents filed in the last 20 years. No claims or full text. Links to other records are by name (and state): EXACT means the identical full name and state; FUZZY means a looser name match, with the confidence shown.