Patents
US 9873746 B2: Methods of synthesizing heteromultimeric polypeptides in yeast using a haploid mating strategy
- Granted
- 2018-01-23
- Filed
- 2011-03-24 application 13070583
- Type
- Utility · 8 claims
- Elsewhere
- Google Patents ↗ · USPTO patent document ↗ · USPTO Patent Center record ↗
Abstract
Methods are provided for the synthesis and secretion of recombinant proteins preferably large mammalian proteins or hetero-multimeric proteins at high levels and for prolonged time in polyploid, preferably diploid yeast. These methods use various mating competent yeast, including Pichia. In a preferred embodiment, a first expression vector is transformed into a first haploid cell; and a second expression vector is transformed into a second haploid cell. The transformed haploid cells, each individually synthesizing a non-identical polypeptide, are identified and then genetically crossed or fused. The resulting diploid strains are utilized to produce and secrete fully assembled and biologically functional hetero-multimeric protein.
Source: PVGPATDIS, file of 2026-04-10 (ipg180123.xml).
Assignees
| Assignee | Type | Place | Also on furp.org | Source |
|---|---|---|---|---|
| KECK GRADUATE INSTITUTE 13 patents | US company or organization | Claremont, CA | Keck Graduate Institute College or university EXACT high confidence: identical full name and state KECK GRADUATE INSTITUTE Nonprofit EXACT high confidence: identical full name and state | PVGPATDIS, file of 2026-04-10 |
| ALDERBIO HOLDINGS LLC 103 patents | US company or organization | Las Vegas, NV | PVGPATDIS, file of 2026-04-10 |
Inventors
| Inventor | Place | Patents | Person page | Source |
|---|---|---|---|---|
| James M. Cregg | Claremont, CA | 14 | PVGPATDIS, file of 2026-04-10 | |
| John Latham | Seattle, WA | 117 | PVGPATDIS, file of 2026-04-10 | |
| Mark Litton | Seattle, WA | 20 | PVGPATDIS, file of 2026-04-10 | |
| Randall Schatzman | Redmond, WA | 19 | PVGPATDIS, file of 2026-04-10 | |
| Ilya I. Tolstrorukov | Claremont, CA | 4 | PVGPATDIS, file of 2026-04-10 |
CPC categories
| Subclass | Category | First group | Source |
|---|---|---|---|
| C07K | Peptides | C07K16/2896 | PVGPATDIS, file of 2026-04-10 |
| C12N | Microorganisms or enzymes; compositions thereof; propagating, preserving, or maintaining microorganisms; mutation or genetic engineering; culture media | C12N15/81 | PVGPATDIS, file of 2026-04-10 |
Cooperative Patent Classification, as currently assigned (one row per subclass).
Assignments and security interests
| Recorded | What | From | To | Reel/frame | Source |
|---|---|---|---|---|---|
| 2021-06-02 | Correction Corrective assignment to correct the assignee name previously recorded at reel: 054161 frame: 0877. assignor(s) hereby confirms the assignment. | LUNDBECK SEATTLE BIOPHARMACEUTICALS, INC signed 2019-11-29 | H. LUNDBECK A/S VALBY | 56449/543 | PASDL, file of 2026-10-15 |
| 2020-09-03 | Assignment Assignment of assignor's interest | LUNDBECK SEATTLE BIOPHARMACEUTICALS, INC signed 2019-11-29 | H. LUNDBECK A/S. VALBY | 54161/877 | PASDL, file of 2026-10-15 |
| 2020-09-03 | Name change Change of name | ALDER BIOPHARMACEUTICALS, INC signed 2019-11-04 | LUNDBECK SEATTLE BIOPHARMACEUTICALS, INC BOTHELL, WA | 53681/224 | PASDL, file of 2026-10-15 |
| 2020-06-23 | Assignment Assignment of assignor's interest | ALDERBIO HOLDINGS LLC signed 2020-03-30 | H. LUNDBECK A/S VALBY | 53020/331 | PASDL, file of 2026-10-15 |
| 2014-02-25 | Assignment Assignment of assignor's interest | ALDER BIOPHARMACEUTICALS, INC. signed 2014-02-24 | ALDERBIO HOLDINGS LLC LAS VEGAS, NV | 32289/791 | PASDL, file of 2026-10-15 |
| 2014-02-07 | Assignment Assignment of assignor's interest | SCHATZMAN, RANDALL signed 2013-12-12 LITTON, MARK signed 2013-12-13 LATHAM, JOHN signed 2013-12-16 CREGG, JAMES M. signed 2014-01-17 TOLSTORUKOV, ILYA I signed 2014-02-07 | ALDER BIOPHARMACEUTICALS, INC. BOTHELL, WA KECK GRADUATE INSTITUTE CLAREMONT, CA | 32172/723 | ECORSEXC, file of 2026-09-25 |
Documents recorded with the USPTO Assignment Recordation Branch that list this patent. A security interest is a lender’s claim on the patent as collateral; a release ends it.
USPTO bulk data: PatentsView granted patents, disambiguated (PVGPATDIS) for patents, assignees, inventors, places and CPC categories; the USPTO Patent Assignment Dataset (ECORSEXC) for assignments and security interests. Patents filed in the last 20 years. No claims or full text. Links to other records are by name (and state): EXACT means the identical full name and state; FUZZY means a looser name match, with the confidence shown.