Patents
US 10683488 B2: System and method for creating crystals of insect acetylcholinesterase
- Granted
- 2020-06-16
- Filed
- 2018-06-25 application 16017503
- Type
- Utility · 16 claims
- Elsewhere
- Google Patents ↗ · USPTO patent document ↗ · USPTO Patent Center record ↗
Abstract
A method of creating crystals of insect acetylcholinesterase. A polynucleotide is obtained that encodes for acetylcholinesterase in a targeted insect. The polynucleotide contains a catalytic core sequence. A recombinant DNA construct is formed by adding a fusion protein and a polyhistidine tag to the catalytic core sequence. The recombinant DNA construct can be further modified by adding known mutations for resistance to insecticides. A growth medium is transfected with the recombinant DNA construct. A polypeptide encoded by the recombinant DNA construct is separated from the growth medium to form a concentrate. The polyhistidine tag is removed from the concentrate. The concentrate is exchanged into a buffer to create a buffered concentrate. Crystals, suitable for X-ray crystallography are then grown with the buffered concentrate.
Source: PVGPATDIS, file of 2026-04-10 (ipg200616.xml).
Assignees
| Assignee | Type | Place | Also on furp.org | Source |
|---|---|---|---|---|
| New York Structural Biology Center 5 patents | US company or organization | New York, NY | THE NEW YORK STRUCTURAL BIOLOGY CENTER, INC. State business registration FUZZY medium confidence: same name (legal endings aside) and state NEW YORK STRUCTURAL BIOLOGY CENTER INC Nonprofit FUZZY medium confidence: same name (governing-board words and legal endings aside) and state | PVGPATDIS, file of 2026-04-10 |
Inventors
| Inventor | Place | Patents | Person page | Source |
|---|---|---|---|---|
| Jonah Cheung | Brooklyn, NY | 2 | PVGPATDIS, file of 2026-04-10 |
CPC categories
| Subclass | Category | First group | Source |
|---|---|---|---|
| C12N | Microorganisms or enzymes; compositions thereof; propagating, preserving, or maintaining microorganisms; mutation or genetic engineering; culture media | C12N9/18 | PVGPATDIS, file of 2026-04-10 |
| A01N | Preservation of bodies of humans or animals or plants or parts thereof ; biocides, e.g. as disinfectants, as pesticides or as herbicides ; pest repellants or attractants; plant growth regulators | A01N63/50 | PVGPATDIS, file of 2026-04-10 |
| C07K | Peptides | C07K7/06 | PVGPATDIS, file of 2026-04-10 |
| C12Y | Enzymes | C12Y301/01007 | PVGPATDIS, file of 2026-04-10 |
Cooperative Patent Classification, as currently assigned (one row per subclass).
Assignments and security interests
| Recorded | What | From | To | Reel/frame | Source |
|---|---|---|---|---|---|
| 2018-06-25 | Assignment Assignment of assignor's interest | CHEUNG, JONAH signed 2018-06-20 | NEW YORK STRUCTURAL BIOLOGY CENTER NEW YORK, NY | 46194/400 | ECORSEXC, file of 2026-09-25 |
Documents recorded with the USPTO Assignment Recordation Branch that list this patent. A security interest is a lender’s claim on the patent as collateral; a release ends it.
USPTO bulk data: PatentsView granted patents, disambiguated (PVGPATDIS) for patents, assignees, inventors, places and CPC categories; the USPTO Patent Assignment Dataset (ECORSEXC) for assignments and security interests. Patents filed in the last 20 years. No claims or full text. Links to other records are by name (and state): EXACT means the identical full name and state; FUZZY means a looser name match, with the confidence shown.